Objective To detect expression of CK20 mRNA in peripheral blood of patients with colorectal carcinoma and its clinical significance. Methods Using the reverse transcriptase-polymerase chain reaction (RT-PCR),CK20 mRNA expression was examined in peripheral blood from 42 patients with colorectal carcinoma before and after operation, 20 healthy volunteers, 20 fresh colorectal carcinoma samples. Results The positive expression rates of CK20 mRNA were 45.24%(19/42) and 33.33%(14/42) before and after operation in 42 colorectal carcinoma patients respectively. All 20 fresh colorectal carcinoma samples revealed expression of CK20 mRNA, but the 20 normal blood samples did not. Conclusion The detection of CK20 mRNA in peripheral blood is helpful to early diagnose, assess the prognosis and make a correct treatment of colorectal carcinoma.
目的探讨手辅助腹腔镜腹部肿瘤切除术手术技术。方法应用手辅助腹腔镜技术结合吻合器及超声切割止血刀完成直肠癌切除1例，乙状结肠癌切除2例，胃壁平滑肌瘤切除1例，胆囊、胆总管结石并左侧腹巨大肠系膜囊肿行胆囊切除、胆总管探查、囊肿切除1例。结果5例患者术后恢复良好，失血量最多为100 ml。结论手辅助腹腔镜技术操作安全，损伤小，将是腹腔镜大器官切除的新方向。