【Abstract】ObjectiveTo eliminate the interference of CEA-related substances in CEA measurement and increase the specificity of CEA in the detection of malignant digestive diseases. MethodsCEA level of peripheral blood and digestive juice (bile, gastric juice) from patients with benign or malignant digestive diseases was measured by ELISA, and semi-dry electrophoretic transfer method of Western blot technique to distinguish CEA and CEA-related substances. ResultsIn malignant diseases, the CEA level of digestive juice was significantly higher than that in the blood, and there was no difference of CEA level in digestive juice and blood in benign diseases. Meanwhile, the CEA level of digestive juice and blood in malignant diseases were significantly higher than that in benign diseases. A specific band (molecular weight about 210×103) was detected in all malignant diseases except four cases whose CEA level was too low (less than 5 μg/L), whereas no one of benign diseases had this specific band no matter how high or low the CEA level was. ConclusionThe specificity of CEA detection in malignant digestive diseases can be improved by using digestive juice as sample and combining with Western blot technique.
目的探讨血清CA153、CA125和CA19-9检测对乳腺癌早期诊断的价值。方法采用化学发光免疫法分别检测乳腺癌、乳腺良性疾病患者及正常健康体检者血清CA153、CA125和CA19-9的水平。结果乳腺癌患者血清的CA153、CA125和CA19-9的表达水平及表达阳性率均明显高于正常对照者和乳腺良性疾病患者(Plt;0.01)； 乳腺癌患者术后CA153、CA125及CA19-9表达水平较术前明显下降(Plt;0.01)，与正常对照者比较差异无统计学意义(Pgt;0.05)。结论肿瘤标志物CA153、CA125及CA19-9 对乳腺癌有一定的诊断价值，且可作为监测乳腺癌病情进展、评估治疗疗效及预后的指标。
Ｎｉｎｅｔｙ ｃａｓｅｓ ｏｆ ｓｕｒｇｉｃａｌｌｙ ｔｒｅａｔｅｄ ｒｅｃｕｒｒｅｎｔ ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ ｉｓ ｒｅｐｏｒｔｅｄ ｗｉｔｈ ａｎａｌｙｓｉｓ ｏｆ ｔｈｅ ｍａｃｈａｎｉｓｍ ｏｆ ｒｅｃｕｒｒｅｎｃｅ ａｎｄ ｆａｃｔｏｒｓ ａｆｆｅｃｔｉｎｇ ｔｕｍｏｒ ｒｅｃｕｒｒｅｎｃｅ． Ｅａｒｌｙ ｄｅｔｅｃｔｉｏｎ ｏｆ ｒｅｃｕｒｒｅｎｃｅ ｄｅｐｅｎｄｓ ｏｆ ＡＦＰ ｍｅａｓｕｒｅｍｅｎｔｓ ａｎｄ ｕｌｔｒａｓｏｎｏｇｒａｐｈｙ ｆｏｌｌｏｗｕｐ ｍｏｎｉｔｏｒｉｎｇ ａｆｔｅｒ ｒｅｓｅｃｔｉｏｎ． Ｈｅｐａｔｉｃ ｒｅｓｅｃｔｉｏｎ ｉｓ ｔｈｅ ｆｉｒｓｔ ｃｈｏｉｃｅ ｏｆ ｔｒｅａｔｍｅｎｔ ｗｈｅｎｅｖｅｒ ｉｔ ｉｓ ｆｅａｓｉｂｌｅ． Ｉｎｔｒａｔｕｍｏｒ ｅｔｈａｎｏｌ ｉｎｊｅｃｔｉｏｎ ａｎｄ ｔｒａｎｓｃａｔｈｅｔｅｒ ａｒｔｅｒｉａｌ ｃｈｅｍｏｅｍｂｏｌｉｚａｔｉｏｎ ｄｕｒｉｎｇ ｏｐｅｒａｔｉｏｎ ｈａｓ ｂｅｅｎ ｂｅｎｅｆｉｃｉａｌ ｉｎ ｓｏｍｅ ｐａｔｉｅｎｔｓ ｗｉｔｈ ｕｎｒｅｓｅｃｔａｂｌｅ ｒｅｃｕｒｒｅｎｔ ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ． Ｔｈｅｓｅ ｒｅｓｕｌｔｓ ｓｕｇｇｅｓｔ ｔｈａｔ ｒｅｏｐｅｒａｔｉｏｎ ｆｏｒ ｒｅｃｕｒｒｅｎｔ ｈｅｐａｔｏｃｅｌｌｕｌａｒ ｃａｒｃｉｎｏｍａ ｍａｙ ｂｅ ａｎ ａｐｐｒｏａｃｈ ｔｏ ｉｍｐｒｏｖｅ ｔｈｅ ｌｏｎｇｔｅｒｍ ｓｕｒｖｉｖａｌ ａｆｔｅｒ ｈｅｐａｔｉｃ ｒｅｓｅｃｔｉｏｎ．