目的 探讨持续性非卧床腹膜透析（CAPD）患者低钾血症的发生及临床特点。 方法 选取2002年12月－2011年12月采取持续非卧床腹膜透析治疗的47例尿毒症患者，就其透析过程中低钾血症发生情况进行总结分析。 结果 低钾血症31例（66%）；低钾血症组与非低钾血症组比较，低钾血症组血尿素氮、肌酐、血钠、血氯、血钙及白蛋白明显降低（P＜0.05）；两组年龄、性别、糖尿病肾病所占的比例及透析龄差异均无统计学意义（P＞0.05）。血钾水平随白蛋白浓度、血氯及血钙的提高而下降（OR=0.756， 0.772，0.022，P＜0.05）。 结论 密切联系、定期随诊观察CAPD患者，提高患者就诊依从性，及时发现与纠正低钾血症，有助于提高CAPD患者的生活质量与长期存活率。
ObjectiveTo observe the clinical effects, advantages, and disadvantages of a new penile circumcision and suturing device in circumcision, by comparing it with traditional circumcision and circumcision with Shang Ring.MethodsThe clinical data of 397 outpatients who underwent surgery for redundant prepuce or phimosis between February 2016 and February 2018 in the Third Affiliated Hospital of Zunyi Medical University were retrospectively collected. The patients chose their surgical types in accordance with the principle of voluntary, with 134 cases undergoing traditional circumcision (the traditional circumcision group), 153 cases undergoing circumcision with Shang Ring (the Shang Ring group), and 110 cases undergoing circumcision with the new penile circumcision and suturing device (PCSD group). The safety, clinical efficacy, and complications among the three groups were compared.ResultsThere were significant differences in postoperative pain scores (F=86.901, P<0.001), edema scores (F=315.656, P<0.001), and appearance scores (F=230.952, P<0.001) among the three groups, and the pain, edema, and appearance scores were significantly better in the PCSD group than those in the traditional circumcision group (P<0.05) and the Shang Ring group (P<0.05). The differences among the three groups in the postoperative pain relief time (F=236.622, P<0.001), intraoperative bleeding volume (F=115.375, P<0.001), edema subsiding time (F=75.614, P<0.001), operation time (F=965.420, P<0.001), and incision healing time (F=42.584, P<0.001) were statistically significant, and the PCSD group was superior to the traditional circumcision group (P<0.05) and the Shang Ring group (P<0.05) in pain relief time, edema subsiding time, and incision healing time. The incidence of postoperative complications in the PCSD group was significantly lower than that in the traditional group and the Shang Ring group (χ2=21.622, P<0.001; χ2=22.778, P<0.001). However, there was no significant difference in intraoperative bleeding volume or operation time between the Shang Ring group and the PCSD group (P=1.000, 0.379), and no significant difference in the incidence of postoperative complications between the traditional circumcision group and the Shang Ring group (χ2=0.014, P=0.912). The proportion of cases undergoing the three surgical methods in each half year changed significantly (χ2=18.721, P<0.001).ConclusionThe use of the new type of penile circumcision and suturing device for circumcision is simple and convenient in clinical operation, with fewer complications, quick recovery, and high satisfaction, and it is worthy of application and promotion.
【Abstract】ObjectiveTo study the effect of down-regulation of E-cadherin on the invasion ability of tumor cells. MethodsHuman pancreatic carcinoma cell line JHP-1 was treated with E-cadherin antisense oligodeoxynucleotied (ASODN). The immunocytochemistry, Western blot were used to detect the expression and the contents of E-cadherin in the tumor cells, and the invasive ability of tumor cells were evaluated by invasive-MTT assay. Results Treated with E-cadherin ASODN, the expression of E-cadherin on JHP-1 cells were reduced, and the protein contents were decreased as well compared with control groups and ODN group. The invasive ability of JHP-1 cells to the basement membrane was increased (P＜0.001) compared with ODN group and control group. ConclusionE-cadherin was related to the invasive ability of tumor cells.
Electrocardiogram (ECG) signals are susceptible to be disturbed by 50 Hz power line interference (PLI) in the process of acquisition and conversion. This paper, therefore, proposes a novel PLI removal algorithm based on morphological component analysis (MCA) and ensemble empirical mode decomposition (EEMD). Firstly, according to the morphological differences in ECG waveform characteristics, the noisy ECG signal was decomposed into the mutated component, the smooth component and the residual component by MCA. Secondly, intrinsic mode functions (IMF) of PLI was filtered. The noise suppression rate (NSR) and the signal distortion ratio (SDR) were used to evaluate the effect of de-noising algorithm. Finally, the ECG signals were re-constructed. Based on the experimental comparison, it was concluded that the proposed algorithm had better filtering functions than the improved Levkov algorithm, because it could not only effectively filter the PLI, but also have smaller SDR value.
ObjectiveTo evaluate the effectiveness of internal fixation with headless compression hollow embedding screws in the treatment of intraarticular fracture of elbow.MethodsBetween March 2012 and September 2018, 12 patients with intraarticular fracture of elbow were treated with internal fixation with headless compression hollow embedding screws. There were 7 males and 5 females with an average age of 50.3 years (range, 22-65 years). Cause of injury included falling in 7 cases, falling from high places in 4 cases, and traffic accident in 1 case. Ten patients were distal humerus fractures which were classified as type 13-B3 in 8 cases and type 13-C3 in 2 cases according to the International Association of Internal Fixation Research (AO/ASIF). Two patients were radial head fractures which were classified as type Ⅲ according to the modified Mason classifications. The preoperative visual analogue scale (VAS) score was 8.25±0.83. The time from injury to operation was 3-5 days (mean, 3.7 days).ResultsAll incisions healed by first intention. All 12 patients were followed up 6-15 months, with an average of 8.4 months. The results of X-ray films and CT examination showed that the fracture ends were anatomic reduction, and the fractures healed at 6-11 months after operation, with an average of 7.8 months. One patient had heterotopic ossification at 4 months after operation. The VAS scores were 5.17±0.79 at 2 weeks after operation and 0.50±0.50 at last follow-up. There were significant differences between the time points (P<0.05). At last follow-up, the Mayo elbow function score was 68-95, with an average of 83.9. The activity of elbow joint recovered.ConclusionThe intraarticular fracture of elbow can be firmly fixed by the headless compression hollow embedding screw, which can allow the early functional training of the elbow joint, reduce the incidence of heterotopic ossification, and obtain good effectiveness.
ObjectiveTo explore the key genes and potential molecular mechanisms of liver and lymph node metastases relevant to duodenal neuroendocrine tumors (DNET). MethodsThe tissues of paracancerous duodenal epithelial, primary lesion, liver metastasis lesion, and lymph node metastasis lesion of a rare DNET accompanied by liver and lymph node metastases were sequenced and analyzed. The differentially expressed genes (DEGs) were screened for different tissues and the functional enrichment analysis was performed. ResultsThe tissues of paracancerous duodenal epithelial was used as the control, a total of 2 053 DEGs expressed only in the liver metastases lesion tissues and 742 DEGs expressed only in the lymph node metastases lesion tissues were screened out, and the top 5 genes expressed in the liver metastases lesion tissues were ORM1, C4BPA, AHSG, C9, and LBP, which in the lymph node metastases lesion tissues were ABHD12B, AC100850.1, HOXC9, AC083967.1, and HOXC8. Kyoto Encyclopedia of Genes and Genomes enrichment analysis found that the DEGs were mainly enriched in the phosphatidylinosiol 3 kinase / protein kinase B pathway, mitogen-activated protein kinase pathway, human papillomavirus infection, etc. ConclusionMultiple DEGs and pathways in metastatic lesions are found in this patient with DNET accompanied by liver metastasis and lymph node metastasis, which provides a new direction for treatment and prophylaxis of DNET.
Objective To analyze the cl inical features of scol iosis associated with Chiari I malformation in adolescent patients, and to explore the val idity and safety of one-stage posterior approach and vertebral column resection for the correction of severe scol iosis. Methods Between October 2004 and August 2008, 17 adolescent patients with scol iosis associated with Chiari I malformation were treated with surgical correction through posterior approach and pedicle instrumentation. There were 9 males and 8 females with an average age of 15.1 years (range, 12-19 years). The MRI scanning showed that 16 of 17 patients had syringomyel ia in cervical or thoracic spinal cord. Apex vertebra of scol iosis were located atT7-12. One-stage posterior vertebral column resection and instrumental correction were performed on 9 patients whose Cobb angle of scol iosis or kyphosis was more than 90°, or who was associated with apparent neurological deficits (total spondylectomy group). Other 8 patients underwent posterior instrumental correction alone (simple correction group). All patients’ fixation and fusion segment ranged from upper thoracic spine to lumbar spine. Results The operative time and the blood loss were (384 ± 65) minutes and (4 160 ± 336) mL in total spondylectomy group, and were (246 ± 47) minutes and (1 450 ± 213) mL in simple correction group; showing significant differences (P lt; 0.05). In total spondylectomy group, coagulation disorder occurred in 1 case, pleural perforation in 4 cases, and lung infection in 1 case. In simple correcction group, pleural perforation occurred in 1 case. These patients were improved after symptomatic treatment. All patients were followed up 24-36 months (32.5 months on average). Bony heal ing was achieved at 6-12 months in total spondylectomy group. No breakage or pull ingout of internal fixator occurred. The angles of kyphosis and scol iosis were significantly improved at 1 week after operation (P lt; 0.01) when compared with those before operation. The correction rates of scol iosis and kyphosis (63.4% ± 4.6% and 72.1% ± 5.8%) in total spondylectomy group were better than those (69.4% ± 17.6% and 48.8% ± 19.3%) in simple correction group. Conclusion Suboccipital decompression before spine deformity correction may not always be necessary in adolescent scol iosis patients associated with Chiari I malformation. In patients with severe and rigid curve or apparente neurological deficits, posterior vertebral column resection would provide the opportunity of satisfied deformity correction and decrease the risk of neurological injury connected with surgical correction.
ObjectiveTo investigate the effect and mechanism of silent information regulator 1 (SIRT1)in invasion of human gastric cancer. MethodsThe expressions of SIRT1 protein and vascular endothelial growth factor A (VEGF-A) protein in 46 cases of gastric cancer were tested by immunohistochemical SP method; the effect of expressions of SRIT1 and VEGF-A protein on prognosis of gastric cancer was analyzed by Kaplan-Meier test; the expressions of SRIT1 and VEGF-A protein in human gastric mucosa GES-1 cells and SGC7901 cells were tested by Western blot method; after the interference of siRNA on SIRT1 gene, expressions of SRIT1 and VEGF-A protein were also tested by Western method, and the invasion ability was determined by Transwell test. ResultsCompared with normal gastric mucosa tissues, expression levels of SIRT1 and VEGF-A protein of gastric tissues were both higher (P < 0.050), and survival situation of patients with SIRT1-positive (P=0.001) or SIRT1-positive and VEGF-A-positive (P=0.006) were both bad, but there was no significant difference on the relationship between prognosis of gastric cancer and expression of VEGF-A protein (P=0.091). Expression levels of SITR1 protein (P=0.010) and VEGF-A protein (P=0.020) in GES-1 cells were both higher than those of SGC7901 cells. In siRNA positive group, expressions of SIRT1 and VEGF-A protein (P=0.010) of SGC7901 cells down-regulated, and invasion ability decreased (P=0.000). ConclusionsSIRT1 gene may promote the expression of VEGF-A protein and the invasion ability of gastric cancer, it may be a therapeutic target of invasion inhibition for gastric cancer.