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《中华眼底病杂志》2006至2008年引文计量分析

Effects of transforming growth factor beta on airway remodeling of obese asthmatic mice treated with pirfenidone

Objective To investigate the effects of transforming growth factor beta (TGF-β) on airway remodeling in obese asthmatic mice and intervention effects of pirfenidone. Methods Seventy-five C57BL/6J mice were randomly divided into five groups, namely a blank control group (group A), an obese group (group B), an obese asthmatic group (group C), a budesonide treatment group (group D) and a pirfenidone treatment group (group E). The mice in the B, C, D, and E groups were fed with high fat diets, then the mice in the C, D, and E groups were sensitized and challenged with ovalbumin (OVA) to establish the model of chronic obese asthma. The mice in group A were fed with normal diets, sensitized and challenged with normal saline. The mice in group D were treated with budesonide (0.5 mg/ml), and the mice in group E were treated with pirfenidone (300 mg/kg). After 4 weeks of treatment, the total number of white cells as well as the percentage of leukocytes, eosinophils, neutrophils and macrophage in bronchoalveolar lavage fluid (BALF) were counted. ELISA and Western blot were used to evaluate the expression of TGF-β. The pathological changes of mice were observed under light microscope by HE and periodic acid-Schiff staining. Meanwhile the remodeling indices were measured including total bronchial wall area (WAt), smooth muscle area (WAm), and bronchial basement membrane perimeter (Pbm). Results The levels of leukocyte and eosinophils in BALF, expression of TGF-β, WAt/Pbm and WAm/Pbm in group C were higher than those in group A, B, D, and E (allP<0.05). The levels of eosinophils in BALF, WAt/Pbm in group E were lower than those in group D (allP<0.05). The level of TGF-β decreased in a sequence of group C>D>E>B>A (allP<0.05). The expression of TGF-β was in a positive correlation with eosinophil percentage in BALF (r=0.79,P<0.01). Conclusions The expression of TGF-β in the airway of obese asthmatic mice is closely related to airway inflammation, airway hyper-secretion and airway remodeling. Pirfenidone can effectively inhibit the expression of TGF-β and improve airway remodeling.

盐酸氨基葡萄糖与双醋瑞因治疗骨关节炎的临床对照研究

目的比较盐酸氨基葡萄糖与双醋瑞因治疗骨关节炎（OA）的疗效及安全性。 方法选取2011年1月－2012年3月120例OA患者，随机分为盐酸氨基葡萄糖组（A组）、双醋瑞因组（B组）和盐酸氨基葡萄糖联合双醋瑞因组（C组），治疗12 周后观察治疗前后临床疗效和安全性。 结果3组患者治疗后较治疗前在20 m步行痛、关节触痛、采用西方安大略和麦克马斯特大学骨关节炎指数评分（WOMAC）的骨关节炎指数和健康状况均有明显改善（P＜0.05）；3组停药1 个月后上述指标与治疗结束时相比差异无统计学意义（P＞0.05）；C组患者改善率优于A、B组（P＜0.05）；3组患者不良反应发生率差异无统计学意义（P＞0.05），均未出现严重不良反应。 结论盐酸氨基葡萄糖与双醋瑞因单用治疗OA疗效和安全性相当，联合应用可取得比单用更好的疗效。