目的 通过分析环境空气中二氧化硫与过敏原的关联性，探讨二氧化硫在评估过敏性疾患中的作用。 方法 收集2005年1月1日－2012年12月31日绵阳市4个监测点二氧化硫浓度，及2007年7月1日－2012年12月31日的过敏性疾病患者各种过敏原的阳性百分比，采用Pearson相关分析探讨二氧化硫浓度变化对过敏原的影响。 结果 2005年－2012年绵阳市二氧化硫年平均浓度分别为（0.060 ± 0.022）、（0.054 ± 0.018）、（0.046 ± 0.012）、（0.030 ± 0.020）、（0.026 ± 0.010）、（0.035 ± 0.012）、（0.036 ± 0.008）、（0.030 ± 0.009） mg/m3。淡水鱼组合（fs34），海鱼组合（fs33），羊肉（f88），黄豆（f14），花生（f13），狗上皮（e2）与二氧化硫的Pearson相关系数分别为：0.144、0.186、0.209、0.150、0153、0.197。检验P值分别为：0.019、0.002、0.001、0.015、0.013、0.001。按α=0.05标准认为与二氧化硫存在正相关关系。而其余的尘螨组合（ds1）、普通豚草（w1）、律草（u80）、树组合（ts20）、雷菌组合（ms1）、蟑螂（i6）、屋尘（h1）、牛肉（f27）、虾（f24）、蟹（f23）、牛奶（f2）、鸡蛋白（f1）、猫毛（e1）、艾蒿（w6），按α=0.05标准，不能认为与二氧化硫存在正相关关系。 结论 环境空气二氧化硫与淡水鱼、海鲜、羊肉、大豆、花生及狗皮屑具有明确的相关性，对树组合、普通豚草、艾蒿、尘螨组合、屋尘、猫毛、蟑螂、霉菌组合、律草、鸡蛋白、牛奶、牛肉、虾、蟹等无明确的相关性。
ObjectiveTo investigate the role of interleukin-25 (IL-25) and its receptor during allergen challenge test in allergic asthmatics as well as its underlining mechanism.MethodsFifteen allergic asthmatic patients with dual response in allergen challenge test were enrolled and blood samples were collected before and after challenge test. The expression levels of IL-25 receptor on the surface of eosinophils, plasma and intracellular IL-25 levels were measured by flow cytometry and enzyme-linked immunosorbent assay. Besides, the function of eosinophils from these patients was evaluated through the expression of type 2 cytokines, degranulation and chemotaxis after stimulation with IL-25.ResultsUpon allergen challenge, the expression of IL-17RB on the surface of eosinophils were increased from (7 426±2 824)/106 white blood cells to (19 446±5 593)/106 white blood cells (P<0.001). The expression of IL-17RA/RB on eosinophils were significantly increased from (4 508±1 360)/106 white blood cells to (9 025±3 166)/106 white blood cells (P<0.001). The plasma level of IL-25 increased from (650±45) pg/ml to (851±43) pg/ml (7 hours after allergen challenge) and (813±56) pg/ml (24 hours after allergen challenge) (P<0.001). The intracellular IL-25 expression of eosinophils was also upregulated from (10 398±1 909)/106 white blood cells to (147 684±46 222)/106 white blood cells (P<0.05). In vitro study, IL-25 (1 ng/ml) stimulated eosinophils for 2 hours promoted its expression of peroxidase [(12.5±4.2) ng/ml compared to control (1.26±0.4) ng/ml, P<0.05). The intracellular expression of IL-5 and IL-13 in eosinophils were also increased after stimulated by IL-25. IL-25 (1 pg/ml) stimulation compared to control could increase eosinophil migration in eotaxin [(36±3) vs. (69±5), P<0.05).ConclusionIL-25 and its receptor play a critical role in eosinophilic aggregation, activation and mobilization during allergic inflammation in allergic asthmatics.