Purpose To estabalish a quantifying model of retinal neovascularization suitable for the study of pathogenesis and therapeutic intervention for the retinal neovascularization. Methods Sixteen one-week-old C57BL/6 mice were exposed to 75% oxygen for 5 days and then to room air and 16 mice of the same age kept in room air as controls.Ink-perfused retinal flatmount was examined to assess the oxygen-induced changes of retinal vessels.The proliferated neovascular response was quantitated by counting the nuclei of endothelial cells of new vessels extending from the retina into the vitreous in 6 mu;m sagittal cross sections. VEGF and bFGF were determined on the cross-sections after immunohistochemcal stain. Results Constriction and closure of the blood vessels were found under the hyperoxia condition,and dilation and proliferation were found under the relatively hypoxia status.There was a mean of 24 neovascular nuclei per cross-section in the oxygen-treated retina and less than 1 nucleus in the control group (P<0.001).VEGF stain was found ber in the inner retinal layer of oxygen-treated mouse than in that of the controls. Conclusion The quantifying model of retinal neovascularization may fascilitate the further researches of medical intervention and pathogenesis of retinal neovacularization. (Chin J Ocul Fundus Dis,2000,16:213-284)
目的:探讨高龄白内障患者术前角膜内皮功能状态。方法:对从2007年11月到2008年10月的66例年龄在80岁或以上的左眼白内障患者术前角膜内皮细胞形态及中央角膜厚度进行回顾性分析。分析参数包括角膜内皮细胞密度,内皮细胞平均面积,最大细胞面积和最小细胞面积,内皮细胞变异系数,面积标准差,六角形细胞比例及角膜中央厚度。结果:患者的角膜内皮细胞平均密度为(2720.3±324.6)个/mm2,平均细胞面积为(375.4±70.3)μm2,最大细胞面积(922.1±243.5)μm2,最小细胞面积(131.2±40.2)μm2,面积标准差(143.5±34.8)μm2,细胞变异系数(38.0±7.3)%,六角形细胞比例(52.8±10.1)%。中央角膜厚度与角膜内皮细胞密度之间无相关性。结论:高龄人白内障患者的角膜内皮细胞密度高,角膜功能基本正常,是保持角膜透明的基础,为眼内手术或治疗提供安全保障。
目的 通过比较白内障患者与正常人用两种方法测量的前房深度和眼轴长度值,观察IOL Master和接触式A型超声测量是否存在差别及其关联程度。 方法 选取2010年12月-2011年2月期间行白内障摘除加人工晶状体植入术的年龄相关性白内障患者及除屈光不正外没有其他眼部病变的志愿者共89例。分别用IOL Maste和A型超声测量54例(96只眼)白内障患者和35例(70只眼)正常者的前房深度(ACD)和眼轴长度(AL),应用配对t检验对每组两种方法测得的ACD及AL值进行比较,并应用Pearson相关分析比较两种方法的相关性。应用独立样本t检验比较白内障组和正常者组间两种方法测得的差值是否不同。 结果 白内障组A型超声和IOL Master测得的ACD值分别是(2.83 ± 0.34)、(3.05 ± 0.39) mm,AL值分别是(23.93 ± 2.46)、(24.27 ± 2.57) mm,差异均有统计学意义(P<0.05);正常者组A型超声和IOL Master测得的ACD值分别是(3.16 ± 0.36)、(3.43 ± 0.46) mm,AL值分别是(24.16 ± 1.61)、(24.49 ± 1.62) mm,差异均有统计学意义(P<0.05)。两种测量方法的相关系数分别是rACD=0.823(P<0.05)和rAL= 0.995(P<0.05)。白内障组和正常者组两种方法测得的ACD差值分别是(0.23 ± 0.23)、(0.28 ± 0.30) mm;AL差值分别是(0.34 ± 0.27)、(0.33 ± 0.15) mm;两组间ACD和AL差值的比较,差异均无统计学意义(P=0.243,0.742)。 结论 不论是白内障组还是正常者组,用IOL Master测得的ACD及AL值均比A型超声测得的相应值高,但是两种方法测得的值高度相关。白内障组和正常者组用两种方法测得的差值相比无差别;在可测到ACD及AL值的情况下,两种测量方法的差值均不受晶状体密度的影响。