【摘要】 目的 探讨慢性缺氧对大鼠岩神经节神经元酸敏感离子通道（acid-sensing ion channels，ASICs）亚型3（ASIC3）和亚型2a（ASIC2a）表达的影响。 方法 将12只健康成年SD大鼠随机分为正常组和缺氧组。用免疫组织化学法（PV）观察正常和慢性缺氧大鼠岩神经节神经元ASIC3和ASIC2a的表达。 结果 给予慢性缺氧刺激后，岩神经节ASIC3阳性表达神经元数目增多（Plt;0.05），灰度值降低（Plt;0.05）；而ASIC2a阳性表达神经元数目和灰度值无明显变化（Pgt;0.05）。 结论 慢性缺氧可上调大鼠岩神经节神经元ASIC3的表达，而对ASIC2a的表达无明显影响，提示ASIC3和ASIC2a可能在岩神经节对缺氧的反应中起着不同的作用。【Abstract】 Objective To investigate the effects of chronic hypoxia on expression of acid-sensing ion channels (ASIC) 3 and ASIC2a in neurons of petrosal ganglions of rats. Methods A total of 12 SD rats were randomly assigned to control group and hypoxia group. The expressions of ASIC3 and ASIC2a of the neurons in the petrosal ganglions in the two groups were investigated with the immunohistochemical technique. Results The level of positive ASIC3 expression in the petrosal ganglions was higher in the hypoxia group than that in the control group (Plt;0.05); the difference of positive ASIC2a expression levels between the control group and the hypoxia group was not statistically significant (Pgt;0.05). Conclusion Chronic hypoxia can significantly increase the expression of ASIC3, but not that of ASIC2a, of the neurons in the petrosal ganglions, suggesting their different roles in mediating a cellular response to chronic hypoxia.
目的:探讨酸敏感离子通道(acid-sensing ion channels，ASICs)的四种亚型即ASIC1a、ASIC1b、ASIC2a和ASIC3是否表达于大鼠岩神经节(petrosal ganglion)神经元。方法:采用常规免疫组化法(PV法)，观察正常大鼠岩神经节神经元是否表达ASIC1a、ASIC1b、ASIC2a和ASIC3。结果:在正常大鼠岩神经节神经元，可见ASIC1a、ASIC1b、ASIC2a和ASIC3表达;ASIC1a与ASIC2a，ASIC3与ASIC1b，ASIC3与ASIC1a，ASIC3与ASIC2a在岩神经节神经元上共表达。结论:正常生理情况下，大鼠岩神经节神经元均表达ASIC1a、ASIC1b、ASIC2a和ASIC3;ASICs亚型之间的共表达提示,在岩神经节可能存在ASICs异聚体的方式。