Objective To investigate the effects of DNA methyltransferase inhibitor (DNMTi) and histone deacetylase inhibitor (HDCAi) on expression of E-cadherin gene and invasiveness of cholangiocarcinoma cell. Methods According to different treatment, the QBC939 cells were divided into four groups: blank control group, hydralazine group, valproic acid group and hydralazine and valproic acid combined group. After 48 h, the expression of E-cadherin was evaluated by reverse transcription-PCR (RT-PCR), mehtylation specific PCR (MSP) and Western blot, the invasiveness of QBC939 cells was evaluated by Transwell method. Results There was no expression of E-cadherin mRNA and protien in blank control group and valproic acid group. The expressions of E-cadherin mRNA and protien in hydralazine and valproic acid combined group were higher than those in hydralazine group ( P ＜ 0.01), while the invasiveness of QBC939 cells of hydralazine and valproic acid combined group was much lower than that of blank control group, hydralazine group and valproic acid group ( P ＜ 0.01). Conclusion DNMTi and HDACi can synergistically re-express E-cadherin gene and weaken the invasiveness of QBC939 cell, which plays an important part in treatment of cholangiocarcinoma.
目的探讨腹腔镜胆囊切除（LC）术中胆管损伤的分型与处理。 方法回顾性分析我院2009年4月至2012年4月期间12例LC并发胆管损伤患者的首次手术过程及对胆管损伤的处理。 结果12例胆管损伤患者中手术中转开腹修补6例，术中未及时发现于术后3周内发现而再次手术6例。3例单纯胆管修补，6例行胆管修复T管支撑引流术，3例行胆管空肠Roux-en-Y吻合术。2例发生了不同程度的术后吻合口狭窄。全组无手术死亡病例。 结论熟悉胆囊三角（Calot三角）脉管变异及病理结构空间改变，熟练的手术操作，必要的中转开腹可减少胆管损伤的发生。损伤发生后及时合理的处理可获良好疗效，普外科医师在操作中应注意。