ObjectiveTo study the relationship between expression of Caspase-3 and regression of hemangioma. MethodsCaspase-3 expression was detected in 79 hemangiomas and 5 normal skin tissues by immunohistochemistry (SP). ResultsIn 79 hemangiomas, the positive rates of Caspase-3 expression in proliferating, involuting and involuted phases were 52.4%, 91.2% and 83.3%, respectively. The expression of Caspase-3 in involuted and involuting phases was higher than proliferating phase(Plt;0.05，Plt;0.01) and control group(Plt;0.01). There was no statistical difference between the latter two groups (Pgt;0.05). ConclusionCaspase3 is involved in the converting of hemangiomas from proliferating phase into involuting phase. The activation of Caspase-3 may induce apoptosis of endothelial cells, thus leading to the regression of hemagioma.
To investigate the diagnosis and treatment of primary colonic malignant lymphoma. Retrospective analysis of 14 cases of primary colonic malignant lymphoma in our hospital from 1983 to 1995. Result: All patients were treated surgically. The resection rate was 93% and the radical operative rate was 69%. Their pathological types were all NHL including 9 cases of B cell lymphoma and 6 cases of T cell lymphoma. 5-year survival rate was 35.7%. Conclusion: grasping of clinical manifestations of this disease is important to diagnosis. The combined therapies with the surgical resection as the first selection are advocated. Immunohistochemistry classification is a sensitive index to prognosis.
To investigate the significance of epidermal growth factor receptor (EGFR) in thyroid carcinoma, the expression of EGFR in 81 samples of thyroid carcinoma were determined by immunohistochemical SP method and comparison among thyroid carcinoma, thyroid adenomas and normal thyroid tissue adjacent to the cancer were made. The results showed: EGFR expression was positive in 45 cases (55.6%) of thyroid carcinoma with no positive expression either in thyroid adenomas or normal thyroid tissue adjacent to the cancer (Ｐ＜0.01). There were no statistical differences between EGFR positive rate and thyroid carcinomatous pathological type, clinical stage, depth of invasion, lymph node metastasis or patients′ postoperative survival time (Ｐ＞0.05). This data suggests that expression of EGFR in thyroid carcinoma is associated with its autonomous growth and malignant phenotype, but it is probably not a useful index for assessing the biological behavious and prognosis of thyroid carcinoma.
Ｂｙ ｕｓｉｎｇ ｍｅｄｉｃａｌ ｉｍａｇｅ ｐｒｏｃｅｓｓ ｓｙｓｔｅｍ ｆｏｒ ＤＮＡ ｃｏｎｔｅｎｔｓ，２６ ｃａｓｅｓ ｏｆ ｔｈｙｒｏｉｄ ｔｕｍｏｒｓ ｔｈａｔ ｃｏｍｐｒｉｓｅｄ ａｄｅｎｏｍａ ５，ｐａｎｉｌｌａｒｙ ａｄｅｎｏｃａｒｃｉｎｏｍａ １４，ｆｏｌｌｉｃｕｌａｒ ｃａｒｃｉｎｏｍａ ５，ｕｎｄｉｆｆｅｒｅｎｔｉａｔｅｄ ｃａｒｃｉｎｏｍａ ２ ａｎｄ ｎｏｒｍａｌ ｔｈｙｒｏｉｄ ｔｉｓｓｕｅ ３ ｗｅｒｅ ｄｅｔｅｃｔｅｄ．Ｔｈｅ ＤＮＡ ｃｏｎｔｅｎｔｓ ｉｎ ｔｈｅ ｎｕｍｂｅｒ ｏｆ ｐｏｌｙｐｌｏｉｄｙ ｗｅｒｅ：ｃａｒｃｉｎｏｍａ ｔｈｅ ｈｉｇｈｅｓｔ ｉｎ ａｍｏｕｎｔ，ａｄｅｎｏｍａ ｔｈｅ ｍｅｄｉｕｍ ａｎｄ ｎｏｒｍａｌ ｔｈｙｒｏｉｄ ｔｉｓｓｕｅ ｔｈｅ ｌｅａｓｔ，ｈｅｎｃｅ ｗｅ ｐｒｏｐｏｓｅ ｔｈａｔ ｔｈｅ ｄｅｔｅｒｍｉｎａｔｉｏｎ ｏｆ ＤＮＡ ｐｌｏｉｄｙ ｉｎ ｔｈｙｒｏｉｄ ｔｕｍｏｒｓ ｍａｙ ｂｅ ｕｓｅｄ ａｓ ａｎ ａｄｊｕｖａｎｔ ｔｏ ｅｖａｌｕａｔｅ ｔｈｅ ｐｒｏｌｉｆｅｒａｔｉｖｅ ａｃｔｉｖｉｔｙ ｏｆ ｔｈｙｒｏｉｄ ｔｕｍｏｒ．
To study the prognostic significance of proliferative cell nuclear antigen(PCNA)in bile duct carcinoma,expression of PCNA protein was studied immunohistochemically in 30 patients with bile duct carcinoma.Results:86.7 percent of bile duct carcinomas showed PCNA positive staining and significiant difference was observed between malignant and benign tissues.These results suggest that proliferative activity of malignant tissues was ber than that of the benign ones.In patients with cancer of stages 3,the mean survial time for those with high proliferative activity was 13 months in constrast with 26 months for those with low activity.PCNA is of prognostic significance for bile duct carcinoma patients.
We have used immunphistochemical SP method to detect the expression of nm23 gene protein in human thyroid tissues from 86 carcinomas, 20 adenomas and 25 carcinomatous adjecent tissues. The results showed the positive staining rate were 73.3%, 40.0%and 16. 0% respective (Plt; 0. 005). Although the expression of nm23 protein had no association with the cervical lymph node metas-tases,it was significantly concordant with the tumor cell diffierentiation (Plt;0. 01) ,tumer capsule (Plt; 0. 05) and TNM stage (Plt;0.05).In addition, the patient‘s average survival time in nm23-positive cases was longer than that in nm23-negative ones (Plt;0.01).This data suggest that nm23 gene may play an important role in thyroid carcinogenesis and the expression of nm23 protein would be an useful marker in assessing the prognosis of the thyroid carcinomas.
The authors observed the progressive changes of the platelet activity in 25 cases of acute cholangitis severe type(ASCT)within 2 weeks of pre-and-post-operation.The results revealed that there are signficant changes of the platelet activity in ASCT.The levels of the platelet activity were proportional to the severity of disease and degree of biliary duct obstruction.Persistency of abnormality of platelet activity may predict the worse of disease and bad prognosis.The results are useful to understand the complex changes of pathophysiology in ACST,to the mechanism of multiple organic failure.