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find Author "陈振兵" 8 results
  • 逆行掌背动脉皮瓣切取方法的改进及应用

    Release date:2016-09-01 09:30 Export PDF Favorites Scan
  • 舟骨月骨间韧带损伤修复早期效果

    目的 评价采用骨锚治疗舟骨月骨间韧带损伤的早期临床效果。 方法 2005 年9 月- 2008 年12 月, 采用Mitek 骨锚修复9 例舟骨月骨间韧带损伤,其中男8 例,女1 例;年龄24 ~ 40 岁,平均31 岁。受伤距韧带修复时间为7 ~ 40 d,平均22 d。9 例经X 线片检查均符合舟骨月骨间韧带损伤诊断标准。术后定期随访,包括疼痛程度、腕关节活动度、双手握力及X 线片检查。腕关节总体功能评价采用Krimmer 腕关节评分表。患者自我功能评价采用患者源性功能调查表上肢功能(DASH)评定表。 结果 患者术后切口均Ⅰ期愈合。9 例均获随访,随访时间7 ~ 18 个月,平均10 个月。术后6 个月X线片检查示舟骨月骨间隙及舟月角分别为(2.8 ± 0.7)mm、(53 ± 9)°,较术前的(5.1 ± 0.8) mm、(80 ±13)° 明显减小,差异有统计学意义(P lt; 0.05)。术后12 个月腕关节屈伸活动度为(97 ± 16)°,术前为(60 ± 10)°;尺桡偏活动度为(55 ± 12)°,术前为(32 ± 9)°;双手握力为(36 ± 7)kg,术前为(28 ± 6)kg;腕关节疼痛视觉模拟评分(VAS)为(21.0 ± 5.2)分,术前为(43.0 ± 11.0)分;以上各指标手术前后比较差异均有统计学意义(P lt; 0.05)。术后18 个月腕关节总功能Krimmer 评分为(82 ± 12)分,其中优4 例,良4 例,中1 例,与术前(56 ± 10)分比较差异有统计学意义(P lt; 0.05)。术后12 个月DASH 评分为(23 ± 12)分,术前为(42 ± 14)分,手术前后比较差异有统计学意义(P lt; 0.05)。 结论 骨锚修复舟骨月骨间韧带能恢复舟月骨稳定性,术后腕关节功能明显改善。

    Release date:2016-09-01 09:04 Export PDF Favorites Scan
  • EFFECT OF EXOGENOUS ERYTHROPOIETIN ON DENERVATED MUSCLE ATROPHY

    Objective To investigate the effect of exogenous erythropoietin (EPO) on the denervated muscle atrophy. Methods Twenty-four SD male rats, weighting 200-220 g were made the models of denervated gastrocnemius muscle after sciatic nerves were transected under the piriform muscle at the right lower leg, and were randomly divided into two groups (n=12). rhEPO (2 500 U/kg) was injected daily into the denervated gastrocnemius muscle in EPO group, and normal sal ine was injected into the denervated gastrocnemius muscle in control group. To observe the general state of health of the experimental animal, the muscle wet weight, the muscle cell diameter, the cross section area, the protein amount, thepercentage of the apoptotic muscle cells, and the Na+-K+-ATPase and Ca2+-ATPase activities were measured 2 and 4 weeks after operation. Results All experimental animals were survived during experiment without cut infection, and all animals could walk with pull ing the right knee. At 4 weeks after operation, 7 cases showed ulcer in the right heel, inculding 5 in the control group and 2 in the EPO group. At 2 and 4 weeks after operation, the muscle wet weight in EPO group was (885.59 ± 112.35) and (697.62 ± 94.74) g, respectively; in control group, it was (760.63 ± 109.05) and (458.71 ± 58.76) g, respectively; indicating significant differences between two groups (P lt; 0.01). The protein amount in EPO group was (77.37 ± 5.24) and (66.37 ± 4.87) mg/mL, respectivly;in control group, it was (65.39 ± 4.97) and (54.62 ± 6.32) mg/mL;indicating significant differences between two groups (P lt; 0.01). At 2 and 4 weeks after operation, the myofibrillar shapes were nearly normal in EPO group while there were muscle fiber atrophy, some collapse and obviously hyperblastosis between muscle bundle. There were significant differences in the muscle cell diameter and the cross section between two groups (P lt; 0.01). However, the percentage of the apoptotic muscle cells was 11.80% ± 1.74% and 28.47% ± 1.81% in control group, respectively, which was significantly smaller than that in EPO group (21.48% ± 2.21% and 55.89% ± 2.88%, P lt; 0.01). At 2 and 4 weeks after operation, Na+-K+-ATPaseand Ca2+-ATPase activities in EPO group were higher than those in control group (P lt; 0.01). Conclusion EPO can delay the denervated muscle atrophy.

    Release date:2016-09-01 09:05 Export PDF Favorites Scan
  • EFFECTS OF NEUROTROPHIC FACTOR 3 GENE MODIFIED SC ON SCIATIC NERVE REGENERATION IN RATS

    Objective To investgate the effects of neurotrophic factor 3 (NT-3) genes modified SC on facil itating nerve regeneration and protecting neuronal survival after the sciatic nerve transection in rats. Methods The double sciatic nerves were harvested from 3-day-old Wistar rats and the SCs were separated, cultured and purified with double enzyem digestion and adherent culture. The third generation purified SCs were used. The NT-3 cDNA gene was transfected into culturedSCs by using cationic l iposome. The NT-3 expression were identified by ELISA after 1, 2, 4 and 8 weeks. The plasmids expressing NT-3 genes were transfected into SCs with l ipofectamine. The purity of SCs were detecting before and after modified with NT-3. The nerve-grafting complexes were constructed by SCs (3 × 107/mL) modified NT-3, third generation SCs (3 × 107/mL), NT-3 gene, respectively. And the nerve-grafting complexes were combined with ECM gel and PLGA conduit. Forty-eight adult SD rats were made the models of the right sciatic nerve defect (10 mm). According to the nerve-grafting complexes which were repaired the sciatic nerve defects, the models were divided into 4 groups randomly (n=12): group A (ECM gel and PLGA conduits), group B (SC, ECM gel and PLGA conduits), group C (NT-3 gene, ECM gel and PLGA conduits) and group D (NT-3 modified SC, ECM gel and PLGA conduits). At 2, 4, 6, 8 and 12 weeks after operation, the nerve gross were observed. Electrophysiological examination, histological observation and transmission electron microscope observation were performed at 12 weeks after operation. Results The concentrations of NT-3 protein were 0.39 ± 0.25, 0.76 ± 0.22, 1.06 ± 0.38 and 1.61 ± 0.35 at 1, 2, 4 and 8 weeks after operation; showing statistically significant differences (P lt; 0.05). The purity of SCs was 94.7% ± 2.1% and 95.6% ± 2.5% before and after modified with NT-3, respectively; showing a statistically significant difference (P lt; 0.05). The feet of injury rats began inflammation and ulcer, which healed at 12 weeks in group D, followed by groups C and B, but which was serious in group A gradually. The observations of gross, sections under microscope and transmission electron microscope at 12 weeks showed the regeneration of defect nerve was best in group D, followed by groups C and B, and group A was worst. There were statistically significant differences (P lt; 0.05) in latent period, ampl itude, motor nerve conduction velocity, the number and thickness of axon, the diameter of nerve fiber, the percentage of the nerve tissue area between group A and groupsB, C, D, between groups B, C and group D at 12 weeks. At 12 weeks after operation, the transmission electron microscope showed observation the maturation of medullary sheath was best in group D, followed by groups C and B, and group A was worst. Conclusion The nerve-grafting complex of NT-3 genes modified SCs could repair injured nerve. The competence is superior to SCs and neurotrophic factors.

    Release date:2016-09-01 09:08 Export PDF Favorites Scan
  • INFLUENCE OF TRANSFORMING GROWTH FACTOR β1 ON DENERVATED MOUSE MUSCLE DERIVED STEM CELL PRODUCING CONNECTIVE TISSUE GROWTH FACTOR AT DIFFERENT TIME POINTS IN VITRO

    Objective To observe the influence of the transforming growth factor β1(TGF-β1) on the denervated mouse musclederived stem cells(MDSCs) producing the connective tissue growth factor(CTGF)at different time points in vitro. Methods MDSCs from the primarycultureof the denervated mouse skeletal muscle were isolated and purified by the preplate technique, and they were identified before the culture and after the culturein vitro with TGF-β1 (10 ng/ml) for 24 hours. Then, MDSCs were randomlydivided into 6 groups (Groups A, B, C, D, E and F) according to the different time points, and were cultured in vitro with TGF-β1 (10 ng/ml) for 0, 3, 6, 12, 24 and 48 hours, respectively. The levels of CTGF mRNA in MDSCs were measured by the real time RT-PCR and the expression of CTGF protein was detected by the CTGF Western blot. Results The immunohistochemistry revealed that before the adding of TGF-β1, MDSCs highly expressed Sca-1, with a positivityrate of 96%; however, after the adding of TGF-β1, the positive expression of Sca-1 decreased greatly, with a negativity rate gt;99%. The Western blot test showed that the ratios of CTGF to the average absorbance of βactin in Groups A-F were 0.788±0.123, 1.063±0.143, 2.154±0.153, 2.997±0.136, 3.796±0.153 and 3.802±0.175, respectively. In Groups AD,the absorbance increased gradually, with a significant difference between the abovementioned groups (Plt;0.05). However, in Groups D-F, there was no significant difference between the groups as the promotive tendency became less significant (P>0.05). The RT-PCR test showed that the △Ct values in GroupsA-F were 1.659±0.215, 1.897±0.134, 2.188±0.259, 2.814±0.263,2.903±0.125 and 3.101±0.186, respectively. In Groups A-D, the increase in the △Ct value was gradual, but the differences were significant between the groups (Plt;0.05). But in Groups E and F, the promotive tendency became less significant(Pgt;0.05). Conclusion TGF-β1 can promote the production of CTGF inthe mouse MDSCs cultured in vitro and the time-dependent relation exists for 3-12 hours.

    Release date:2016-09-01 09:23 Export PDF Favorites Scan
  • 上肢功能评定表

    Release date:2016-09-01 09:33 Export PDF Favorites Scan
  • 逆行指动脉岛状皮瓣修复手指皮肤缺损21例

    Release date:2016-09-01 11:07 Export PDF Favorites Scan
  • 第3掌背动脉逆行岛状皮瓣切取方法的改进

    Release date:2016-09-01 11:04 Export PDF Favorites Scan
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