Objective To observe the histological changes and apoptosis of retinal cells in pigmented rabbits treated by transpupillary thermotherapy (TTT) with different laser power. Methods Fourteen pigmented rabbits (28 eyes) were divided averagely into seven groups（control group, 50, 70, 90, 110, 130, and 150 mW group）according to different laser power of TTT. Light microscopy was performed to observe the histological changes, and TDT-mediated biotin-dUTP nick-end labeling (TUNEL) technique and flow cytometry (FCM) examination were used to detect the apoptotic cells 24 and 48 hours after photocoagulation, respectively. Results The color of retinal burn speckles changed from offwhite to white and super white with the diameter enlarged gradually as the laser power of TTT increased. The results of light microscopy revealed that compared with the control group, the retinal tissue did not change much in 50-70 mW group; in 90-130 mW group, the retinal structure was integrated, but the cone and rod cells became swollen and condensed nuclei and cytoplasmic vacuolization were seen in the inner nuclear layer. The difference of retinal structure in 50-130 mW group 24 and 48 hours after photocoagulation and control group was not significant. In 150 mW group, tumefaction and degeneration were observed in each layer of retina and the inner and outer segments of photoreceptor cells lost 24 hours after photocoagulation, and obvious necrosis and cell loss of retinal tissues were detected hours after photocoagulation. The results of TUNEL examination indicated that positive cells were found in outer nuclear layer in each photocoagulation group which increased as the laser power of TTT was enhanced; the apoptosis gradually involved the inner nuclear layer and ganglion cell layer. The results of flow cytometry (FCM) examination showed the peak of apoptotic cells in each photocoagulation group 24 hours after photocoagulation. Conclusion Under certain subthreshold photocoagulation (50-70 mW), retinal tissue of rabbits does not change much but apoptosis of photoreceptor cells increase significantly. As the laser power of TTT increases, the retinal tissues become swollen, degenerated and even necrotic; cellular apoptosis gradually involves the inner nuclear layer and ganglion cell layer. (Chin J Ocul Fundus Dis, 2006, 22:249-252)
目的 了解新生儿患者多重耐药菌社区感染的特点和定植情况，采取预防控制措施，防止在院内传播。 方法 对2011年9月－2012年8月所有新入院新生儿患者共801例进行耐甲氧西林金黄色葡萄球菌（MRSA）、耐万古霉素肠球菌（VRE）和产超广谱β内酰胺酶（ESBL）菌入院筛查，了解多重耐药菌社区感染的特点和定植情况。并将801例新生儿患者（观察组）医院感染发生率与2010年9月－2011年8月同期801 例新生儿患者（对照组）医院感染发生率进行比较。 结果 观察组发现MRSA和产ESBL菌共321例，检出率为40.1%。其中包括单纯MRSA 45例，占14.1%；产ESBL菌238例，占74.1%；MRSA+产ESBL菌38例，占11.8%。观察组医院感染发生率为2.0%，多重耐药菌医院感染构成比为12.5%；对照组医院感染发生率为5.1%，多重耐药菌医院感染构成比为53.6%；两组医院感染发生率和多重耐药菌医院感染构成比差异均有统计学意义（P＜0.01）。 结论 新生儿患者多重耐药菌定植情况严重，应引起高度重视，加强管理可防止在医院传播，减少医院感染发生。
目的 探讨腹腔镜胆囊切除术（LC） 中采用提胆囊壶腹、看Rouviere沟、胆囊后三角入路法切除胆囊，对成功完成LC和预防胆管、血管损伤的临床意义。方法 2009年7月至2011年12月期间应用提胆囊壶腹、看Rouviere沟、从胆囊后三角入路法（研究组） 切除胆囊112例，同期常规方法（对照组） 行LC 55例，比较2组的临床疗效。结果 2组一般临床资料比较差异无统计学意义（P＞0.05），具有可比性。研究组112例成功完成了LC，无中转开腹及并发症发生。对照组55例LC发生并发症3例，其中2例胆管损伤及1例左肝动脉损伤。研究组的并发症发生率明显低于对照组〔0 （0/112）比5.45% （3/55），P＜0.05〕，研究组和对照组间术中出血量、手术时间、住院时间及中转开腹率差异均无统计学意义 〔术中出血量： （8.95±3.95） mL比（8.09±3.91） mL，P=0.189；手术时间：（84.72±37.15） min比（77.05±37.22） min，P=0.212；住院时间：（6.40±2.39） d比（7.02±3.41） d，P=0.178〕。结论 在LC中，采用提胆囊壶腹、看Rouviere沟、从胆囊后三角入路法，相对容易精准解剖壶腹部与胆囊管移行区，完整显露胆囊壶腹部、胆囊管、胆总管及胆囊动脉，既是成功完成LC的保证，又可有效避免术中胆管和血管的损伤。