目的 总结开展腹腔镜下经胆囊管胆总管探查取石的手术技巧及应用体会。方法 回顾性分析笔者所在医院肝胆外科2010年7月至2012年12月期间行腹腔镜经胆囊管胆总管探查取石术的36例患者的临床资料。结果 30例病例经胆道探条扩张胆囊管后直接完成网篮取石，3例经胆囊管汇入胆总管处微切开完成取石，3例经胆道镜联合激光碎石完成手术。全组病例的手术时间为（110.88±25.99） min，术后住院时间为（6.59±1.18） d，均无严重操作相关并发症发生。结论 腹腔镜下经胆囊管胆总管探查取石术是安全可行的，若能综合各种技术，可提高手术的成功率。
Objective To investigate the role of KiSS-1 gene in the metastatic process of carcinoma of gallbladder and the clinicopathologic significance of KiSS-1 gene expression in carcinoma of gallbladder. Methods Pathological specimens from 59 gallbladder carcinoma tissues (13 hepatic invasion and 13 lymphatic invasion tissues were included), matched with 7 para-tumor and 6 normal gallbladder tissues, were examined for the expression of KiSS-1 gene by tissue microarray technique and immunohistochemistry (EnVision). Results The positive rate of KiSS-1 expression was down-regulated (P＜0.05) in tumor tissues, as compared with normal and para-tumor tissues. In carcinoma of gallbladder, the expression of KiSS-1 had no relationship with the gender, age, tumor size, histological grade or differentiation, and metastasis of lymph node, while was associated with the depth of infiltration, invasion of liver and the clinical stages (Nevin). In Ⅰ+Ⅱ, Ⅲ+Ⅳ and Ⅴ stage, the positive rates of KiSS-1 were 92.3%, 57.1% and 27.8% respectively, with an undeniably clear lowering tendency (P=0.002). Conclusion Down-regulating expression of KiSS-1 is closely associated with the processes of genesis, invasion and metastasis in carcinoma of gallbladder, and may participate in regulating these processes.