ObjectiveThis study construct a pulmonary fibrosis model in vivo to study anti-pulmonary fibrosis effect of ampelopsis.MethodsWe constructed a pulmonary fibrosis model by bleomycin in BALB/c mice. The mice were divided by weight random number table into a blank control group, a model control group, a dexamethasone treatment group (intervened with dexamethasone in a dose of 2.5 mg/kg), and three ampelopsis treatment groups intervened with ampelopsis in dose of 200, 100, and 50 mg/kg, respectively. Bleomycin solution (3 mg/kg) was intratracheally injected respectively on 1st and 14th day, except the blank group. Twenty-eight days later, the relevant indicators were collected, including respiratory function (airway resistance, dynamic lung compliance, maximal ventilator volume), level of hydroxyproline and histopathological changes in the lungs.ResultsAfter 28 days, the model control group mice had severe respiratory resistance, dynamic lung compliance and maximal ventilator volume were decreased. The high dose ampelopsis treatment could enhance respiratory function (P<0.05). Lung coefficient was lower in the treatment groups than that in the model control group (P<0.05). The hydroxyproline of the treatment groups was less than that of the model control group (P<0.05). Histopathological examination showed that the degree of fibrosis increased in the model control group (P<0.05), but decreased in the treatment groups (P<0.05).ConclusionAmpelopsis can resist bleomycin-induced pulmonary fibrosis in mice, relieve the symptoms of respiratory failure, reduce the formation of collagen, and produce anti-pulmonary fibrosis effect.
Objective To explore the correlation between the imaging features of peripheral ground-glass pulmonary nodules and the invasion degree of lung adenocarcinoma, and the high risk factors for infiltrating lung adenocarcinoma under thin-slice CT, which provides some reference for clinicians to plan the surgical methods of pulmonary nodules before operation and to better communicate with patients, and assists in building a clinical predictive model for invasive adenocarcinoma. MethodsClinical data of the patients with peripheral ground-glass pulmonary nodules (diameter≤3 cm) in thin-slice chest CT in the First Affiliated Hospital of Soochow University from January 2019 to January 2020 were continuously collected. All patients underwent thin-slice CT scan and thoracoscopic surgery in our center. According to the pathological examination results, they were divided into two groups: an adenocarcinoma lesions before infiltration group, and an invasive lung adenocarcinoma group. The thin-slice CT imaging parameters of pulmonary nodules were collected. The nodular diameter, mean CT value, consolidation tumor ratio (CTR), nodular shape, vacuolar sign, bronchial air sign, lobulation sign, burr sign, lesion boundary, pleural depression sign, vascular cluster sign and other clinical data were collected. Univariate and multivariate analyses were conducted to analyze the independent risk factors for the infiltrating lung adenocarcinoma, and to analyze the threshold value and efficacy of each factor for the identification of infiltrating lung adenocarcinoma. Results Finally 190 patients were enrolled. There were 110 patients in the adenocarcinoma lesions before infiltration group, including 21 males and 89 females with a mean age of 53.57±10.90 years, and 80 patients in the invasive lung adenocarcinoma group, including 31 males and 49 females with a mean age of 56.45±11.30 years. There was a statistical difference in the mean CT value, nodular diameter, CTR, gender, smoking, nodular type, nodular shape, vacuolar sign, lobulation sign, burr sign, lesion boundary, pleural depression sign, vascular cluster sign between the two groups (P＜0.05). However, there was no statistical difference between the two groups in age (P=0.081), lesion site (P=0.675), and bronchial air sign (P=0.051). Multiple logistic regression analysis showed that nodular diameter, mean CT value, CTR and lobulation sign were independent risk factors for differentiating preinvasive adenocarcinoma from invasive adenocarcinoma. At the same time, the threshold value was calculated by Youden index, indicating that the CTR was 0.45, the nodal diameter was 10.5 mm and the mean CT value was –452 Hu. Conclusion In the peripheral ground-glass pulmonary nodules, according to the patient's CT imaging features, such as mixed ground-glass nodules, irregular shapes, vacuoles, short burrs, clear boundaries, pleural indentations, and vascular clusters, have a certain reference value in the discrimination of the invasion degree of ground-glass pulmonary nodules. At the same time, it is found in this research that peripheral ground-glass pulmonary nodules with diameter greater than 10.5 mm, CT value greater than –452 Hu, CTR greater than 0.45 and lobulation sign are more likely to be infiltrating lung adenocarcinoma.