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find Author "黎银潮" 5 results
  • Bioinformatics analysis of gene expression in Mesio-temporal lobe epilepsy

    ObjectiveTo investigate the significant genes in Mesio-temporal lobe epilepsy (MTLE) and explore the molecular mechanism of MTLE.MethodsThe microarray data of MTLE were downloaded from the Gene Expression Omnibus (GEO) database and analyzed by bioinformatics methods using GEO2R tool, Venny2.1.0, FUNRICH and Cytoscape software, DAVID and String databases.ResultsOf all the 331 differentially expressed genes(DEGs), 46 genes were down-regulated and 285 genes were up-regulated in dataset GSE88992; Furthermore, the core module genes were identified from those DEGs, which were expressed mostly in plasma membrane and extracellular space; The major molecular funtion were chemokine activity, cytokine activity and chemokine receptor binding; The main biological pathways involved neutrophil chemotaxis, inflammatory response and positive regulation of ERK1 and ERK2 cascade; The KEGG analysis showed DEGs enriched in Chemokine signaling pathway, Cytokine-cytokine receptor interaction and Complement and coagulation cascades. In addition, ten hub genes (Il6, Fos, Stat3, Ptgs2, Ccl2, Timp1, Cd44, Icam1, Atf3, Cxcl1) were found to significantly express in the MTLE.ConclusionThe pathogenesis of MTLE involves multiple genes, and multiple cell signaling pathways. Thus investigations of these genes may provide valuable insights into the mechanism of MTLE.

    Release date:2020-07-20 08:13 Export PDF Favorites Scan
  • 超极化激活环核苷酸门控通道在颞叶癫痫的研究新进展

    超极化激活环核苷酸门控通道(Hyperpolarizationactivated cyclic nucleotide gatedchannel,HCN)属于电压门控型离子通道,迄今为止发现有四个亚型:HCN1~HCN4。HCN 通道的激活依赖于膜的超级化,在膜电位低于静息电位时,HCN 通道被激活,产生局部紧张性电流,导致持续的钠内流,使细胞膜发生去极化。该通道分布在人体的分布并不一致,主要在神经系统和心脏中表达。目前研究表明,HCN 通道既参与所在组织的正常生理功能,如睡眠和觉醒、学习和记忆、视觉和疼痛感知、神经元起搏、树突整合等,也与多种中枢神经系统疾病及所在组织的病理状态密切相关,如神经病理性疼痛、学习记忆障碍、药物成瘾和颞叶癫痫,特别是在伴海马硬化性内侧颞叶癫痫中。癫痫作为神经系统最常见的神经疾病之一,癫痫因其病因错综复杂,病理改变亦多样性,至今尚未能完全了解其全部发病机制。目前有大量的文献报道 HCN 与癫痫,特别是颞叶癫痫的发生发展有密切关系。因此本文就 HCN 通道的结构特征、分布、功能、调控及其在颞叶癫痫发生过程中的新研究进展进行综述。

    Release date:2020-03-20 08:06 Export PDF Favorites Scan
  • Bioinformatics analysis of HCN1 gene and protein in human

    ObjectiveTo lay a theoretical foundation for the research of regulation of Hyperpolarization activated cyclic nucleotide gated channel 1 (HCN1) gene expression and its involvement in the pathogenesis of Mesio-temporal lobe epilepsy (MTLE) and other related diseases, the bioinformatics methods were used to analyze sequence characteristic, transcription factors and their binding sites in the promoter region of human HCN1 gene, and the physicochemical properties, signal peptides, hydrophobicity, transmembrane regions, protein structure, interacting proteins and functions of HCN1 proteins.MethodBiological software and website, such as Protparam, Protscale, MHMM, SignalP 5.0, NetPhos 3.1, Swiss-Model, Promoter 2.0, AliBaba2.1 and EMBOSS were used to analyze and predict physicochemical properties, structural functions, localized expression, phylogenetic relationships and protein interactions with human HCN1 protein, and promoter, CpG island and transcription factor characteristics of HCN1 gene.ResultsThe evolutionary analysis of HCN1 protein showed that the genetic distance between human and Pongo abelii was the smallest, indicating the closest genetic relationship between human and Pongo abelii. Human HCN1 protein was an unstable hydrophilic protein located on the plasma membrane, which contained two transmembrane structure. However, the predicted results showed that there was no signal peptide and nuclear localization sequence in this protein. The secondary structure of HCN1 protein was mostly random coil and alpha helix, and it contained multiple potential phosphorylation sites. The ontology analysis results of HCN1 protein were showed as follows. The cellular component of HCN1 protein was located in the plasma membrane (GO:0005886); the molecular functionof HCN1 protein were cyclic adenosine monophosphate binding (GO:0030552) and voltage-gated ion channel activity (GO:0005244); the biological process of this protein were reacting to cAMP (GO:0071320) and transmembrane transport of potassium (GO:0071805). The analysis results of String database showed that the proteins that had close interaction with human HCN1 protein mainly included the ten proteins (HCN2, HCN4, PEX5L, MARCH7, KCTD3, GNAT3, SHKBP1, KCNQ2, FLNA and NEDD4L). These proteins were mainly involved in regulation of ion transport and transmembrane transport of potassium (GO:0071805). The HCN1 gene was located at 5p12 and contained 8 exons and 7 introns.There were at least three promoter regions in the nucleotide sequence of 2 000 bp from the upstream of the HCN1 gene to the 5 'flanks, and contained a 158 bp CpG island in the promoter region and one TATA boxes and one CAAT boxes in the 5' regulation region ofHCN1 gene; niceteen transcription factors, including NF-κB, NF-1, AP-1, TBP, IRF-1, c-Ets-1, Elf-1, HNF-3, HNF-1, YY1, GATA-1, RXR-α, GR, AP-2αA, ENKTF-1, C/EBPβ, C/EBPα, c-Fos and c-Jun, binding in the promoter region of the HCN1 gene were predicted by both softwares (AliBaba2.1 and PROMO2).ConclusionThe analysis results provide important information for further studies on the role of HCN1. Bioinformatics analysis of the promoter region can improve the research efficiency of gene promoters, and provide theoretical basis for subsequent experiments to construct expression vectors of HCN1 gene promoters and identify their functions.

    Release date:2020-09-04 03:02 Export PDF Favorites Scan
  • 基因启动子甲基化在癫痫的研究进展

    DNA 甲基化是人类发现最早的表观遗传学修饰之一,具有多种调控功能,参与机体发育过程中干细胞生长、细胞增殖、器官发育、衰老和肿瘤发生等多个生物学过程,而且在突触重塑、神经细胞分化等神经生物过程中也具有重要作用。近年来,越来越多的研究表明 DNA 甲基化修饰与癫痫的发病机制密切相关,特别是基因启动子的甲基化改变逐渐受到关注。文章主要对表观遗传中基因启动子区的甲基化在癫痫发生发展中的研究进展进行综述。

    Release date:2020-09-04 03:06 Export PDF Favorites Scan
  • Effect of valproic acid coadministred with lamotrigine on epileptic patients' ammonia

    ObjectiveTo investigate the effect of valproic acid (VPA) coadministred with lamotrigine (LTG) on epileptic patients' ammonia and evaluate the influencing factors of elevated blood ammonia in epileptic patients.MethodsA retrospective analysis of clinical data from 146 patients with epilepsy (including newly diagnosed epilepsy patients) who were admitted to the Seventh Affiliated Hospital of Sun Yat-Sen University from May 2018 to April 2020 was performed. The patients were divided into no antiepileptic drug group (group A), VPA group only (group B) and VPA combined LTG group (group C), and the concentration of the blood ammonia of the patients were analyzed.ResultThe average ammonia levels in groups A, B and C were (18.14±1.19), (25.89±0.87) and (36.60±4.34) μmol/L, and the incidence of blood ammonia higher than normal were 2.77%, 8.89% and 20.0%, respectively.The difference between group B and group A and group C were statistically significant (P<0.05), the difference between group C and group A was statistically significant (P<0.05).ConclusionPatients with epilepsy who use VPA were at increased risk of blood ammonia and LTG can increase ammonia in epileptic patients who were treated with VPA. So when VPA was combined with LTG, more attention should be paid to ammonia of patient to avoid adverse reactions.

    Release date:2020-09-04 03:06 Export PDF Favorites Scan
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