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find Author "AN Shucai" 1 results
  • DNA Methylation on Regulation of Cell Apoptosis and Proliferation in Ischemia-Reperfusion of Small Intestine

    Objective To investigate the role of DNA methylation on regulation of cell apoptosis and proliferation in ischemia-reperfusion of small intestine. Methods Thirty-five male Wistar rats were randomly divided into normal group, sham operation group, and ischemia-reperfusion group. The apoptotic cell was assessed by TUNEL and electron microscopy and the expression of Ki-67 was examined by immunohistochemistry in the small intestinal parts (villi epithe-lium, crypt epithelium, and lamina propria mucosa of small intestine). The DNA methylation was detected by DNA histo-endonuclease-linked detection of methylated DNA sites. Results ①The apoptotic positive cells increased at 3 h, 6 h,and 12 h after ischemia-reperfusion in the villi epithelium, crypt epithelium, and lamina propria mucosa of small intestine as compared with the normal group and sham operation group (P<0.01);Moreover, the apoptotic cells in the lamina propria mucosa of small intestine were identified as T cells by electron microscopy. ②The expressions of Ki-67 markedly increased at 3 h, 6 h, 12 h, and 24 h after ischemia-reperfusion in the villi epithelium cells as compared with the normal group and sham operation group (P<0.01). ③The weak expression of DNA methylation was found in the villi epith-elium and crypt epithelium in the normal group and sham operation group, the b expression was examined in the crypt epithelium cells nearby stem cell site in the ischemia-reperfusion of small intestine, the change of expression was gradually weak from crypt epithelium to villi epithelium. Conclusion This initial results indicate that the DNA methyl-ation in the ischemia-reperfusion of small intestine might regulate cell apoptosis and proliferation.

    Release date:2016-09-08 10:23 Export PDF Favorites Scan
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