The present study was designed to elucidate the role of apoE polymorphism in the lithogenesis of cholecystolithiasis and to explore the hereditary pathogenesis of the disease. Polymerase Chain Reaction (PRC) was used as researching apoE phenotypes and allele frequencies in patients with gallstones (n=87) and in controls (n=50), and the fasting serum lipids of subjects were also measured. The characteristics of lipid variants were analysed among the patients with different apoE phenotypes. The results showed that the levels of TG (1.43mmol/L), VLDL-C(0.68mmol/L) in E2/3 patients were greatly higher than those in E2/3 controls (1.06mmol/L, Ｐ＜0.05 and 0.48mmol/L, Ｐ＜0.05), and LDL-C (1.41mmol/L) was markably lower in E2/3 patients than that in controls (2.04mmol/L, Ｐ＜0.05). The levels of serum lipids decreased significantly in E3/3 patients with HDL-C (0.89mmol/L), HDL2-C (0.49mmol/L), HDL3-C (0.39mmol/L), and compared with those in E3/3 controls (1.28mmol/L Ｐ＜0.05, 0.73mmol/L Ｐ＜0.001 and 0.55mmol/L Ｐ＜0.001). In E3/4 patients there were only slight changes of VLDL-C, LDL-C level. The results suggest that the average level of serum lipids in the same apoE phenotype patients with gallstones is higher than that in controls, and the different apoE phenotypes patients with gallstones have different characteristics of dyslipidemia. ε2 allele is probably one of the dangerous factor in the lithogenesis of cholecystolithiasis.
Objective To evaluate the relationship between genetic polymorphism of ApoE and Alzheimer’s disease in Chinese population. Methods Such databases as PubMed, EBSCO, CNKI, CBM, and WangFang Data were searched from their establishment to December 2010 to collect the literature about the relationship between genetic polymorphism of ApoE and Alzheimer’s disease in Chinese population. RevMan 5.0 was adopted to conduct consistency check and data merging, and to evaluate publication bias. Results ApoEε4 was the risky allele (Plt;0.05) in Chinese population, and its pooled odds ratios and 95%CI was 3.53 (2.49 to 5.00). ApoEε3 was the protective alleles (Plt;0.05) in Chinese population, and its pooled odds ratios and 95%CI was 0.52 (0.40 to 0.68). ApoEε4/ε4, ApoEε4/ε3, and ApoEε4/ε2 were the risky genotypes (all Plt;0.05) in Chinese population, and their pooled odds ratios and 95%CI were 10.17 (4.25 to 24.19), 2.57 (2.04 to 3.25), and 1.94 (1.13 to 3.34), respectively. ApoEε3/ε3 was the protective genotype (Plt;0.05) in Chinese population, and its pooled odds ratios and 95%CI was 0.67 (0.57 to 0.77). Conclusion In Chinese population, some ApoE alleles and genotypes are associated with Alzheimer’s disease.
ObjectiveTo systematically review the correlation between apolipoprotein E (ApoE) polymorphism and sporadic Alzheimer's disease (SAD) in Chinese population. MethodsThe case-control studies about the relationship between ApoE polymorphism and SAD in Chinese population were electronically retrieved in PubMed, EMbase, CBM, The Cochrane Library (Issue 8, 2013), CNKI, VIP, and WanFang Data from the date of their establishment to August 2013. Literature screening according to the inclusion and exclusion criteria, data extraction and methodological quality assessment of the included stuides were completed by two reviewers independently. Meta-analysis was then conducted using Stata 12.0 software. ResultsA total of 50 case-control studies invovling 3 396 cases and 4 917 controls were finally included. The results of meta-analysis showed that, in Chinese, the risk of SAD was 2.89 times higher in population with allele ε4 than in population with allele ε3 (OR=2.89, 95%CI 2.61 to 3.19, P < 0.001); 7.24 times higher in those with ε4/ε4 genotype than in those with ε3/ε3 genotype (OR=7.24, 95%CI 5.11 to 10.24, P < 0.001); 2.90 times higher in ε3/ε4 genotype than in ε3/ε3 genotype (OR=2.90, 95%CI 2.56 to 3.29, P < 0.001); 2.11 times higher in ε2/ε4 genotype than in ε3/ε3 genotype (OR=2.11, 95%CI 1.64 to 2.72, P < 0.001); and no statistic significance was found in the risk of SAD compared ε2/ε3, ε2/ε2 genotypes and ε2 allele with ε3/ε3 genotype and ε3 allele. ConclusionFor Chinese population, ApoE allele ε4 is significantly associated with the onset of SAD, and genotype ε4/ε4 is a high risk factor of SAD. While allele ε2 is not associated with the onset of SAD. Since a great deal of current studies failed to conduct stratified analysis, it is suggested to further conduct relevant relevant studies according to clinical classification of SAD and patients' characteristics.