ObjectiveTo investigate the correlation of X-ray repair cross-complementing gene 1 (XRCC1-Arg399Gln, Arg280His, and Arg194Trp) polymorphisms and susceptibility to gastric cancer. MethodsOne hundred and twenty patients with gastric cancer were included in study group, 120 healthy volunteers were included in control group. The DNA was extracted from peripheral blood. Arg399Gln, Arg280His, and Arg194Trp gene polymorphisms were detected and analyzed using polymerase chain reaction-restriction fragment length polymorphism technique, and the susceptibility between different genotypes and gastric cancer was compared in two groups. ResultsThe age, gender, smoking, drinking, diet, and other common characteristics of exposure factors had no significant differences in two groups (P > 0.05). The mutation locus genotype frequencies of Arg399Gln and Arg280His had no significant differences between two groups (P > 0.05). However, the mutation locus genotype frequencies of Arg/Trp, Trp/Trp, and Arg/Trp+Trp/Trp were higher and the mutation locus genotype frequency of Arg/Arg was lower in the study group as compared with the control group (P < 0.05). ConclusionThe preliminary results from this study shows that XRCC1 Arg399Gln and Arg280His polymorphisms are not correlated with susceptibility to gastric cancer; However, Arg194Trp polymorphism is correlated with susceptibility to gastric cancer.
目的探讨DNA损伤修复基因XRCC1 Arg194Trp位点多态性与结直肠癌易感性的关系。 方法选取120例结直肠癌患者与120例正常对照者进行对比研究。取外周血提取DNA，采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）技术对XRCC1 Arg194Trp基因多态性进行检测分析，比较不同基因型与结直肠癌易感性的关系。 结果2组观察对象在年龄、性别、吸烟、饮酒、饮食特点等常见暴露因素方面的差异均无统计学意义（P＞0.05），变异基因型Arg/Trp＋Trp/Trp出现频率在2组观察对象中分别为30.00%和24.17%，差异无统计学意义（P＞0.05）。 结论XRCC1 Arg194Trp位点多态性与结直肠癌的易感性并无显著相关性。