【Abstract】 Objective To establ ish a three-foot weight-bearing canine model to imitate the biomechanical loading environment of the human’s hip joint. To observe and compare the kinetic changes of hind l imbs between normal and three-foot weight bearing canines. Methods Using 10 beagles, three-foot weight-bearing canine models were made by fixing unilateral wrist joints at 90º flexionally. The changes of ground reaction forces and the time of standing phases (Ts) of the hind l imbs were compared by 3-D gait analysis pre- and postoperatively. Results Canines could walk well with three l imbs after the fixation of one fore l imb. However, the gait pattern changed tremendously. The canine walked jumpily by raising its head and neck, and the bilateral hind l imbs kept contacting ground alternately. Ts of ipsilateral hind l imb was (0.48±0.04)s, and Ts of contralateral hind l imb was (0.46±0.06)s. Although, the time durations were increased a l ittle, but there were no significant differences when compared with that of normal canines (0.43±0.05)s (P gt; 0.05). The vertical ground reaction force (Fz) of ipsilateral hind l imbwas (4.63±0.85) body weight, and the Fz of contralateral hind l imb was (4.78±0.49) body weight. There were significant increases when compared with the Fz of normal canines (3.26±0.48) body weight (P lt; 0.05). The peak acceleration force of the ipsilateral hind l imb was (0.80±0.30) body weight. There was significant increase compared with that of normal canines (0.72±0.13) body weight (P lt; 0.05). The peak acceleration force of the contralateral hind l imbs was (0.68±0.22) body weight, there was no difference compared with that of normal canines (P gt; 0.05). The peak deceleration forces of the ipsilateral and contralateral hind l imbs were —(0.26±0.14) body weight and —(0.13±0.05) body weight separately. They decreased significantly when compared with that of normal canines —(0.43±0.13) body weight (P lt; 0.05). In normal canines, the upper l imbs were main load bearingl imbs, they could bear 62.8%±2.4% of body weight. However, the hind l imbs could bear only 37.2%±1.8% of body weight. On the contrary, in three-foot weight-bearing canines, the hind l imbs became the main load bearing l imbs, they could bear 59.1%±6.7% of body weight. Conclusion Three-foot weight-bearing canine model can be used as a candidate animal model to research the effects of biomechanical loading on the progression of hip joint diseases.