Objective To review the latest research progress on keratinocyte growth factor (KGF), to thoroughlyunderstand its basic characteristics and appl ication methods and to lay a sol id foundation for the research and development of new KGF medicines and improving the qual ity of skin substitutes. Methods Domestical and international l iteratures on KGFin recent years were extensively reviewed and analyzed. Results KGF was secreted by mesenchymal cells and its receptors were distributed in epithel ium to promote the prol iferation, migration and differentiation of epithel ial cell specifically, which closely related to the organ development, wound heal ing, tumorigenesis and immune reconstruction. Conclusion KGF can be used to improve wound heal ing and the performance of skin substitutes. However, the structure of KGF needs to be changed to el iminate its side effects and purify its promoting effect on epithel ial cell growth.
To harvest human keratinocyte growth factor (KGF) mimic peptides with Ph.D.-7TM phage display peptide l ibrary. Methods Ph.D.-7TM phage display peptide l ibrary was biopanned for 4 rounds to harvest monoclonal anti-body human KGF and then phage tilter was detected. ELISA detection was performed to detect the binding force of random-selected monoclonal phages, thereafter DNA extracted from phages with better binding activity was sequenced and the Basic BLAST system was appl ied to conduct the sequence similarity and homology analysis. Results After 4 rounds ofbiopanning, the titer of phages was increased gradually and the enrichment of specific phage mimic peptides was obtained. The titers of monoclonal phages were up to 2.0 × 1014 pfu/mL according to ELISA detection. According to the absorbance value, the monoclonal phages with better binding activities to certain specific antibodies were sequenced, and 26 base sequences related to the promotion of division and growth were verified, 2 of which were similar to human KGF. Homology sequence analysis revealed that the common sequence of those 26 base sequences was similar to the partial sequences of human KGF. Conclusion The phage mimic peptides resembl ing or related to human KGF DNA can be harvested from Ph.D.-7TM phage display peptide l ibrary, which may be conducive to improve human KGF performance, wound heal ing and the qual ity of tissue engineered skin substitutes.