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find Keyword "CD4阳性T淋巴细胞" 3 results
  • Effects of mensenchymal stem cell on infiltration of CD4+ T cell subsets in the eyes of experimental autoimmune uveitis

    Objective To observe the therapeutic effect of mensenchymal stem cells (MSCs) for experimental autoimmune uveitis (EAU). Methods MSCs were obtained from Wistar rats and selected by plastic adherence. Lewis rats were divided into treatment group and control group, six rats in each group. EAU models were induced by immunization with an emulsion (0.2 ml) containing 30 mu;g interphotoreceptor retinoid-binding protein derived peptide R16 and complete Freundprime;s adjuvant. The clinical manifestations of two groups were observed. Nine to 11 day after modeling, 1 ml MSCs suspension, which contained 5times;106 MSCs, were injected into the rats in treatment group via tail vein, and the rats in control group were given equal volume of phosphate buffer solution. Fifteen day after modeling, the eyes were collected to test the proportion of interferon gamma;, interleukin-17 and Foxp3 positive cells by flow cytometry. The clinical scores were analyzed by mixed linear model and statistical analysis of variance of repeated measurement data. The results of flow cytometry were analyzed using independent-sample t test. Results Six days after immunization, mild dilatation and congestion of iris vascular was observed. Nine days after immunization, mild muddy anterior chamber, myosis and absent pupillary reaction to light were observed. Twelve days after immunization, muddy anterior chamber, occlusion of pupil and dimmed or disappeared red reflex were observed, and then inflammation was slowly reduced. From 11 to 15 days after immunization, the clinical score of treatment group was lower than that in control group, the difference was statistically significant (t=2.42, 2.21, 4.16, 5.24, 4.03; P<0.05). The results of flow cytometry showed that MSCs treatment could decrease the proportion of CD4+T cells, Th1 cells and Th17 cells, increase the proportion of Treg cells. Conclusion MSCs treatment can ameliorate EAU, up-regulate the expression of Treg cells and down-regulate the expression of CD4+T cells, Th1 cells and Th17 cells.

    Release date:2016-09-02 05:21 Export PDF Favorites Scan
  • 干扰素-α对Beçhet病患者外周血中白介素-17表达的影响

    Release date:2016-09-02 05:26 Export PDF Favorites Scan
  • Th17细胞在葡萄膜炎中的作用

    Th17细胞是新近发现的CD4+辅助性T细胞亚群。Th17细胞分泌以白细胞介素(IL)-17为主的致炎因子,导致炎症产生和升级,在介导多种炎症性自身免疫反应中发挥关键作用;在葡萄膜炎的发病过程中也占有重要地位。有关Th17细胞在葡萄膜炎中的作用机制和信号传导通路已成为免疫学研究的热点,随着Th17细胞的诱导分化、生物效应及其与葡萄膜炎致病关系研究的不断深入,有希望为葡萄膜炎的防治对策开辟新的研究方向和思路。

    Release date:2016-09-02 05:41 Export PDF Favorites Scan
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