ObjectiveTo obtain the mesenchymal stem cells (MSCs) from human umbilical cord and mark in vitro, for further transplantation therapy. MethodsThe MSC were isolated from human umbilical cord by tissue explants culture method. After subculture in vitro, the morphology of hUC-MSC was observed; the surface antigens of hUC-MSC were detected by flow cytometry; adipogenic and osteogenic differentiation were determined by specific staining; hUC-MSC labelled with Brd U were identified by immunofluorescence. ResultsMSC could be isolated successfully by tissue explants culture method. When cultured about one week, the cells climbed out from the tissue block edge, proliferated and formed colonies; the hUC-MSCs of passage 5 were detected by flow cytometry, and they highly expressed CD73, CD90 and CD105, didn't express or lowly expressed CD14, CD34, CD45, CD79a and human leukocyte antigen-DR. After two weeks of adipogenic induction, they were positive in oil red O staining, and after three weeks of osteogenic induction, red precipitate could be seen by alizarin red staining, and the red fluorescence of the hUC-MSC labelled with Brd U could be detected by immunofluorescence detection. ConclusionThe cells can be isolated from human umbilical cord by tissue explants culture method, with the characteristics of hUC-MSCs and can be labeled successfully in vitro, so it can be used for the research in the field of cell transplantation.
Objective To probe into the influence of hospital-community seamless recovery mode on quality of life in schizophrenia patients. Methods Fifty-six patients with stable condition discharged from hospital in 2011 were recruited. All the patients accepted hospital-community seamless recovery mode based on rehabilitation inside the hospital. Personal and social performance scale (PSP) and family APGAR (adaptability, partnership, growth, affection, resolve) index were used to evaluate the change of social function and family APGAR index at the beginning of the study, 3 months and 6 months after this research. All the data were analyzed by SPSS 16.0 software. Results Scores of APGAR scale and PSP scale of patients 3 months and 6 months after rehabilitation training were significantly higher than those before the training (P<0.05). APGAR scale and PSP scale had positive correlation. As APGAR score increased, PSP score also increased (P<0.05). Conclusion Hospital-community seamless recovery mode can improve the quality of life and promote rehabilitation positively in patients with schizophrenia.
Objective To investigate the risk factors of postoperative urinary retention of non-small cell lung cancer patients undergoing video-assisted thoracoscopic surgery (VATS) lobectomy without indwelling urinary catheterization. Methods In this prospective trial, we recruited 148 patients who were scheduled for lung cancer lobectomy under general anesthesia by VATS in Department of Thoracic Surgery in West China Hospital from July through December 2015. These patients were divided into two groups including a trial group and a control group. There was no indwelled urethral catheter in the trial group. And the patients in the control group were indwelled urethral catheter routinely. Postoperative urinary retention, urinary tract infection, the postoperative hospitalization duration and the clinical data were recorded. Results There was no significant difference between the trial group and the control group in postoperative urinary retention (9.46% vs. 6.76%, P=0.087). However, the ratios of the male patients and the patients with history of abdomen operation, and international prostate symptom score (IPSS) of the urinary retention patients (83.33%, 33.33%, 26.55±7.00) were statistically higher than those of the patients without urinary retention (56.62%, 0.00%, 15.31±8.31, P=0.017, P=0.000, P=0.031). Postoperative urinary tract infection rates in the trial group and the patients with urinary retention (4.05%，25%) were statistically higher than those in the control group and the patients without urinary retention (1.35%, 0.74%, P=0.049, P=0.048). Conclusion The risk factors of postoperative urinary retention patients with non-small cell lung cancer undergoing VATS lobectomy are male patients, history of abdomen operation, and moderate to severe hyperplasia of prostate.