ObjectiveTo analyze intrahepatic vascular structures, divide liver segment automatically, and carry out virtual anatomic hepatectomy for virtual liver surgery planning based on liver threedimensional images. MethodsThe branches of portal vein were labeled in the level of segment based on spiral CT scanning images by using the liver surgery planning system software Liv 1.0. Thus, the simulation of an anatomical resection could be carried out. ResultsAccording to the portal segment branches, each individual liver segment could be divided automatically. The three-dimensional liver model was reconstructed based on liver segments, and the margin of liver segments was displayed clearly. On that basis, the simulation of anatomical resection and the volumetric estimation could be performed. ConclusionsThe three-dimensional liver model with intrahepatic vessel is reconstructed clearly, automatic segmentation of liver segment, the simulation of anatomical resection, and volumetric estimation can be applied in succession. The planning can be accomplished with Liv 1.0 on personal computer by the user, which provides a software platform for clinical application of virtual liver techniques.