Objective To study the effects of neonatol rabbit Schwann cells(SC) on repair of optic contusion in adult rabbits. Methods 24 h after the adult rabbit optic nerves was contused,0.1 ml of SC suspension (group A) and saline water (group B) were injected into the vitreous of injured eyes respectively.All the animals were studied by retinal ganglion cell (RGC) and axon counting,flash visual evoked potential (FVEP) tests at various intervals after injury. Results At the 4th week after injury,the number of RGC was (19.89plusmn;3.79)/mm in group A and (12.67plusmn;4.12)/mm in group B,and the density of axons was (94.569plusmn;793)/mm2 in group A and (36.085plusmn;285)/mm2 in group B.There was dramatical difference between group A and B (Plt;0.01).The amplitude of FVEP wave of group A increased from 48% to 88% on the 3rd day after injury,and still dept 78% at the 8th week and group A was significantly higher than group B at various intervals (Plt;0.01). Conclusion SC are effective in promoting the repair of optic nerve contusion by increasing the survival rate of RGC,rescuing axons from degeneration,and dramatically promoting the function of the optic nerve. (Chin J Ocul Fundus Dis,2000,16:91-93)
Purpose To study the effects of Schwann cells(SC) on promoting and supporting axon growth of rabbit retinal neurons in vitro. Methods The scistic nerves of neonatal rabbits were dissected and cultured for 2 weeks to obtain SC monolayers. The retinal cells that had been freshly dispersed were seeded respectively onto the SC monolayers or poly L lysine covered dishes,and the morphology of cultured retinal neurons was observed and the 24th hours and 48th hours respectively under the phase contrast microscopic. Results Retinal neurons of neonatal rabbits attached to the two substrate and extended axons at the 24th hour.Neurite length on SC reached 85plusmn;17mu;m at the 24th hour and 283plusmn;27mu;m at the 48th hour respectively and was significantly longer than on acellular substrate (Plt;0.01) Conciusion SCs are effctive in promoting and supporting neurite growth of retinal neurons in vitro. (Chin J Ocul Fundus Dis,1998,14:212-214)
Diabetic macular edema (DME) is the main cause of vision loss in diabetic patients, its pathogenesis is complicated, and the clinical treatment is not good. DME is extremely harmful to vision. With the deepening of relevant studies, its related pathological mechanism has become more and more clear, and the treatment methods have also changed accordingly. In recent years, the rapid development of intracocular fluid cytokine detection technology has provided a more reasonable explanation of the mechanism of DME and made the choice of treatment more reasonable. However, the acquisition of intraocular fluid is an invasive operation with a certain risk of infection. If the level of relevant cytokines in intraocular fluid can be linked with the relevant imaging indicators, it will provide a better choice for the treatment and prognosis monitoring of DME and reduce the risk of invasive operation, and further clinical studies are needed to explore its correlation in the future.