In order to inquire into the relation between bacterial L-forms and chronic appendictis,appendices removed from 10 patients with appendicitis were cultured with improved kagans’s solid medium and nutrient medium containing blood respectively.Bacterial forms with bacterial L-form transforms to L-forms and 1 strain of bacillus aerogen with its L-form.The result shows that bacterial form transforms to L-form,then to bacterial form may be related with appendicitis attack,alleviation and attack again.