Objective To explore the feasibility and clinical effect of the nano-carbon particles in laparoscopic operation for adenocarcinoma of esophagogastric junction. Methods From 2008 to 2011， 119 patients with adenocar-cinoma of esophagogastric junction who underwent the laparoscopic operation were divided into study group （n=56） and control group （n=63）. The nano-carbon particle was injected into the subserosa around the tumor using the injection needle made by ourselves for lymphatic tracing before the laparoscopic operation in the study group， while no tracer was given in the control group. The indexes of lymph nodes， operation time， intraoperative blood loss， and postoperative hospital stay were compared in two groups. Results The nano-carbon particle was injected into the subserosa around the tumor successfully in the study group. The numbers of dissected lymph nodes and metastatic lymph nodes in the study group were significantly more than those in the control group （dissected lymph nodes：20.52±4.51 versus 16.44±3.80， t=5.341，P=0.000；metastatic lymph nodes：8.88±3.15 versus 6.49±2.49， t=4.602， P=0.000）. There were no statistical diff-erences in the intraoperative blood loss， operation time， and postoperative hospital stay in two groups 〔intraoperative blood loss：（97.50±27.52） ml versus （96.03±22.83） ml， t=0.318， P=0.751；operation time：（221.07±24.25） min versus （230.48±38.54） min， t=-1.570， P=0.119；postoperative hospital stay （10.82±1.67） d versus （10.29±1.33） d， t=1.945， P=0.054〕. Conclusions Injection of the nano-carbon particles using the injection needle made by ourselves is feasible in laparoscopic operation for adenocarcinoma of esophagogastric junction. It can increase number of dissected lymph nodes without increasing operation time， intraoperative blood loss， and postoperative hospital stay.