Objective To analyze the relationship between the collateral ligament attachment and the epicondylar axis with rotational alignment of the femoral component in the total knee arthroplasty(TKA).Methods Twenty normal cadaver knee joints were anatomized and 2 holes were drilled on the distal femur from the deep and superficial insertions of the medial collateral ligaments to the lateral condylar part, respectively. Then, all the knees were scanned by MRI on the sagittal plane, making the drilled hole located relatively to the posterior condylar joint surface on the axial plane, and the posterior condylar angle (PCA) and thecondylar twist angle (CTA) were measured.Results The colateral ligament had the deep and superficial parts, and the deep part was strained during the knee flexing. PCA and CTA were 4.50±1.26° and 7.10±0.30° respectively, and there was a significant difference between them(P＜0.05), which were significantly greater than those reported abroad. On the sagittal plane, there wasno significant difference between the radiuses of the posterior medial and lateral condylar circles (Pgt;0.05). The distance from the center of the posterior condylar circle to the deep insertion of the medial collateral ligament (MCL) (d1) was 4.22±0.20 mm, and the distance to the superficial insertionof MCL (d2) was 7.36±0.13 mm. The difference between d1 and d2 was significant(Plt;0.05). Conclusion The center of the posterior condylar circle passes from the deep insertion of the collateral ligament, which can be regarded as a fixed flexionextension axis of the knee. By releasing the different parts of the collateral ligaments, the balance of the flexion and extension gap canbe obtained, and then varus, valgus or flexed contracture deformity of the kneecan be realigned. Besides, the rotational orientation of the femoral prothesis can be made by a reference to the epicondylar insertion of the collateral ligament.