Objective To observe the effect of vitrectomy (PPV) combined with silicone oil filling on the stability of the tear film. Methods A total of 72 eyes of 36 patients with vitreous hemorrhage and retinal detachment were enrolled in the study with PPV combined with silicone oil filling. The operation and contralateral eyes were set up in the operation group and the control group respectively, each had 36 eyes. The tear film rupture time (BUT), the base tear secretion test or Schirmer Ⅰ test (SⅠT) and corneal fluorescein staining (CFS) were performed at 7, 30, 60, and 90 days after operation. The difference of BUT, SⅠT and CFS at different time points after the operation of the two groups were compared. Results After operation 7, 30 days, SⅠT and CFS increased, BUT staining is shortened in the surgery group, the differences were statistically significant (t=1.78, P＜0.05); after operation 60, 90 days, SⅠT, CFS, BUT were same between the surgery group and the contralateral eyes (t=12.39, P＞0.05). Conclusion PPV combined with silicone oil filling can affect the stability of the tear film, which can be recovered to the preoperative level at postoperative 60 days.