ObjectiveTo analyze the status quo, problems and weak points of cleaning compliance in the Intensive Care Unit (ICU), and assess the intervention effects by evaluating the object surface cleaning quality in the ICU. MethodsBetween September 1st and December 1st, 2014, fluorescence marker was used to mark the surfaces of medical instruments and objects in the ward which were supposed to be cleaned by the nursing and cleaning staff. The assessment of cleaning compliance was performed through observing the residual fluorescence. Then, targeted intervention was carried out for situations with a low cleaning compliance. ResultsBefore the intervention, the thorough cleaning rates of medical instruments in the Comprehensive ICU, Neurological ICU (NICU), and Chest ICU were respectively 43.3%, 31.4%, and 23.8%, and the thorough surface cleaning rates for those units in order were 67.1%, 60.5%, and 48.4%, respectively. After the first intervention, the cleaning rate of medical instruments in the Comprehensive ICU was 47.1%, which had no significant change (P=0.345), but the rate in the NICU and Chest ICU reached respectively 65.3% and 35.1%, which was significantly improved (P<0.05). The object surface cleaning rates were 73.3% and 58.1% in the Comprehensive ICU and Chest ICU after the first intervention, and there was no significant difference compared with those before the intervention (P>0.05), but the object surface cleaning rate in the NICU was significantly improved to 85.5% (P<0.05). After the second intervention, the medical equipment cleaning rates were 66.9%, 83.3%, and 57.4%, respectively for those three units, and compared with those before intervention, all the three were significantly improved (P<0.05). The object surface cleaning rates for NICU and Chest ICU were significantly raised to 85.6% and 84.2% (P<0.05), while it was 65.7% in the Comprehensive ICU and was not significantly improved (P=0.767). ConclusionObservation and supervision through a feedback system can raise the cleaning compliance, which is helpful in controlling and preventing nosocomial infection.