ObjectiveTo explore the learning process, critical steps and complication prevention of heterotopic abdominal heart transplantation (HAHT) model in rats,and effectively improve the learning process and shorten the learning curve. MethodsSurgical experience of 146 rats of HAHT from October 2012 to January 2013 was summarized. Operation time,successful rate and failure reasons were analyzed. ResultsA training time of 140-150 hours was needed to successfully master surgical skills of HAHT in rats. Average operation time was 83±27 minutes. There were 105 successful HAHT rats (72%) and 41 failed HAHT rats(28%) among 146 HAHT rats. Major failure reasons included hemorrhagic shock (16 rats,39%) grafted heart rebeating failure (7 rats,17%) and anastomotic stenosis (7 rats,17%). ConclusionVascular anastomosis is the key procedure for the establishment of HAHT model in rats.
ObjectiveTo study the effect of combined treatment using pancreatic kinionogenase enteric-coated tab and mecobalamin injection on diabetic peripheral neuropathy (DPN) patients. MethodsWe collected 84 subjects with DPN who received treatment from January 2012 to December 2012 in our hospital, and we randomly divided them into treatment group (42 subjects, using pancreatic kinionogenase enteric-coated tab and methylcobalamin injection) and control group (42 subjects, using mecobalamine only). Subjects in the treatment group were given oral pancreatic kininogenase at 120 unit/times and 3 times/day, and methylcobalamin intravenous injection at 1 mg/day for 14 days. Subjects in the control group were only given methylcobalamin intravenous injection at 1 mg/day for 14 days. ResultsIn the treatment group, 22 subjects showed excellent, 19 subjects effective and 1 subject ineffective outcome. In the control group, 8 subjects showed excellent, 22 effective and 12 ineffective outcome. The difference between the two groups is statistically significant (P<0.01). Compared with no treatment, the nerve conduction velocity in both the two groups has been improved. The improvement in the treatment group is significantly better than that of the control group (P<0.01), and all of them had no obvious adverse reaction during the treatment. ConclusionThe combined treatment using pancreatic kinionogenase enteric-coated tab and methylcobalamin injection on DPN is better than using methylcobalamin only.
ObjectiveTo investigate the relationship between thyroid stimulating hormone (TSH) and the blood lipid level in patients with subclinical hypothyroidism (SCH). MethodsWe carried out a retrospective analysis on the clinical data of 264 patients with their first diagnosis of subclinical hypothyroidism without treatment from 2010 January to 2014 January. A total of 288 healthy controls were chosen from communities. The patients were groups based on TSH≥10.0 mU/L and 3.6 mU/L≤ TSH< 10.0 mU/L. We investigated the relationship between TSH and the level of blood lipids by analyzing liver and renal function, blood lipids, thyroid function, and thyroid peroxidase antibody (TPO-Ab) in the patients. ResultsTriglyceride (TG) and high density lipoprotein cholesterol levels were not significantly different among the three groups (P>0.05). Total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) levels of the group with TSH≥10 mU/L were respectively (5.93±1.12) and (3.82±1.11) mmol/L, which were significantly higher than those in the controls[(4.43±1.12) and (2.66±0.43) mmol/L] (P<0.05). TC, TG and LDL-C levels of the group with 3.6 mU/L≤ TSH< 10.0 mU/L were higher than the controls, but the differences were not significant (P>0.05). After thyroid hormone replacement therapy within 12 weeks, TG, LDL-C, and TC levels of TPO-Ab positive patients with subclinical hypothyroidism (n=112) were respectively (4.62±1.03), (2.97±0.52), and (1.17±0.62) mmol/L, which were significantly lower than those levels before treatment[(5.43±1.18), (3.62±0.58), and (2.03±0.71) mmol/L] (P<0.05). ConclusionThe disorder of lipid metabolism exists in patients with subclinical hypothyroidism. Especially, the level of TSH greater than or equal to 10 mU/L is a high risk factor for dyslipidemia. In TPO-Ab positive patients, therapy of thyroid hormone replacement can effectively improve the blood lipid abnormalities in patients with subclinical hypothyroidism, and it may be an effective measure to improve the disorder of lipid metabolism economically and effectively.
ObjectiveTo investigate the association between local population's lifestyle and the morbidity of cerebral stroke in Ganzi Tibetan state, so as to provided references for preventing stroke in the local region. MethodsA representative population sample (including residents, farmers and herdsmen) of Kangding, Dege, Ganzi, Litang and Batang county was selected through randomized cluster sampling from September 2010 to June 2012. Data including lifestyle, housing conditions and stroke status were collected using a questionnaire. Then statistic analysis was performed using SPSS 19.0 software. ResultsA total of 7 038 cases were investigated, of which 125 cases with cerebral stroke were found. The morbidity of stroke was 1 923/100 000. Smoking, alcohol drinking, excessive intake of salt and overweight were positively associated with the risk of cerebral stroke, while appropriate physical exercise was negatively associated with cerebral stroke. Housing conditions and height above sea level were not obviously associated with cerebral stroke. ConclusionThe prevalence of cerebral stroke is high in Ganzi Tibetan state, which is related to special local population's lifestyle. It is very important to reinforce the work for the prevention and control of stroke.