目的:分析基底细胞癌中P53蛋白的表达情况,探讨P53蛋白的表达与基底细胞癌发生发展的关系,以及在基底细胞癌发病机制中的作用。方法:选取2006年7月至2007年2月,北京朝阳医院皮肤科手术切除的基底细胞癌及正常皮肤组织石蜡标本各17例,免疫组织化学法分析P53蛋白在基底细胞癌和正常皮肤组织中的表达情况。结果:17例基底细胞癌中P53蛋白的表达率为100%。综合染色强度和阳性细胞所占比例进行平均半定量分析,P53蛋白在基底细胞癌中表达程度为,而在正常皮肤组织中表达程度为+,两者之间存在统计学上显著性差异。结论:P53在基底细胞癌中呈现高度表达状态,提示P53蛋白的表达与基底细胞癌的发病过程关系密切,可能在其发生发展过程中起重要作用。
Objective To investigate antimicrobial resistance profiles of carbapenem-resistant Enterobacter spp. (CREn) and the bactericidal effects of aztreonam combined with avibactam. Methods The CREn strains isolated from the West China Hospital of Sichuan University between 2016 and 2021 were identified by gyrB gene amplification and subsequent sequencing. The drug sensitivity results, sample types and distribution of relevant patient departments of these strains were summarized. Colistin-resistant and -intermediate strains were selected to carry out the bactericidal test of colistin and aztreonam combined with avibactam. Results A total of 110 clinical strains of CREn were included. The most common strain was Enterobacter xiangfangensis (91 strains), the highest proportion was in the intensive care unit (27.27%), and the proportion of respiratory tract samples was more than 40%. The antimicrobial sensitivity results showed that CREns were all resistant to carbapenems, the resistance rate to colistin was 23.64%, and the resistance rate to aztreonam combined with avibactam was 0. Among other antimicrobial agents, the antimicrobial resistance rate of amikacin and tigecycline were less than 10%. The time-kill curve showed that for colistin-intermediate strains, colistin could achieve bactericidal effect in a shorter time than aztreonam combined with avibactam. However, whether the strain was resistant to colistin or not, the bactericidal rate of 2 μg/mL aztreonam combined with avibactam in 24 hours could exceed 99%. Conclusion CREn is resistant to most commonly used clinical antibacterial drugs, but remains sensitive to aztreonam combined with avibactam, and aztreonam combined with avibactam has bactericidal effect on it.