目的:探讨高龄白内障患者术前角膜内皮功能状态。方法:对从2007年11月到2008年10月的66例年龄在80岁或以上的左眼白内障患者术前角膜内皮细胞形态及中央角膜厚度进行回顾性分析。分析参数包括角膜内皮细胞密度,内皮细胞平均面积,最大细胞面积和最小细胞面积,内皮细胞变异系数,面积标准差,六角形细胞比例及角膜中央厚度。结果:患者的角膜内皮细胞平均密度为(2720.3±324.6)个/mm2,平均细胞面积为(375.4±70.3)μm2,最大细胞面积(922.1±243.5)μm2,最小细胞面积(131.2±40.2)μm2,面积标准差(143.5±34.8)μm2,细胞变异系数(38.0±7.3)%,六角形细胞比例(52.8±10.1)%。中央角膜厚度与角膜内皮细胞密度之间无相关性。结论:高龄人白内障患者的角膜内皮细胞密度高,角膜功能基本正常，是保持角膜透明的基础,为眼内手术或治疗提供安全保障。
Prolonged disorders of consciousness (pDOC) are pathological conditions of alterations in consciousness caused by various severe brain injuries, profoundly affecting patients’ life ability and leading to a huge burden for both the family and society. Exploring the mechanisms underlying pDOC and accurately assessing the level of consciousness in the patients with pDOC provide the basis of developing therapeutic strategies. Research of non-invasive functional neuroimaging technologies, such as functional magnetic resonance (fMRI) and scalp electroencephalography (EEG), have demonstrated that the generation, maintenance and disorders of consciousness involve functions of multiple cortical and subcortical brain regions, and their networks. Invasive intracranial neuroelectrophysiological technique can directly record the electrical activity of subcortical or cortical neurons with high signal-to-noise ratio and spatial resolution, which has unique advantages and important significance for further revealing the brain function and disease mechanism of pDOC. Here we reviewed the current progress of pDOC research based on two intracranial electrophysiological signals, spikes reflecting single-unit activity and field potential reflecting multi-unit activities, and then discussed the current challenges and gave an outlook on future development, hoping to promote the study of pathophysiological mechanisms related to pDOC and provide guides for the future clinical diagnosis and therapy of pDOC.