As the most important refraction part in the optical system, cornea possesses characteristics which are important parameters in ophthalmology clinical surgery. During the measurement of the cornea in our study, we acquired the corneal data of Orbscan Ⅱ corneal topographer in real time using the Hook technology under Windows, and then took the data into the corneal analysis software. We then further analyzed and calculated the data to obtain individual Q-value of overall corneal 360 semi-meridian. The corneal analysis software took Visual C++6.0 as development environment, used OpenGL graphics technology to draw three-dimensional individual corneal morphological map and the distribution curve of the Q-value, and achieved real-time corneal data query. It could be concluded that the analysis would further extend the function of the corneal topography system, and provide a solid foundation for the further study of automatic screening of corneal diseases.