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find Keyword "Human insulin like growth factor-1 receptor" 1 results
  • Establishment of Hepatocellular Carcinoma Cell Lines with Stable Decreased Expressing IGF1R Gene with siRNA

    Objective To establish hepatocellular carcinoma (HCC) cell lines which olig-expressed IGF1R gene stably. Methods An eukaryotic expressing vector pSUPER-IGF1R-siRNA that could block IGF1R expressing was transferred into hepatocellular carcinoma cell lines SMMC7721 and Hep3B with Lipofectamine 2000 reagents. After transferred, cells were selected with G418 to obtain positive clones. The expressions of IGF1R, cyclin D1 and cyclin B1 were detected by RT-PCR and Western-blot. Cell growth curve were painted. Results Two cell lines clones were screened olig-expressing IGF1R gene stably. The experimental cell lines grew more slowly than control cell lines and the expression of cyclin D1 decreased (P<0.05). Conclusion The HCC cell lines for olig-expressing IGF1R gene stably are established successfully.The plasmid pSUPER-IGF1R-siRNA can inhibit the growth of SMMC7721 and Hep3B cell lines, and the expression of cyclin D1.

    Release date:2016-08-28 04:08 Export PDF Favorites Scan
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