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find Keyword "Human leukocyte antigen DRB1 Sequencing-based typing Single-tube amplification" 1 results
  • Single-Tube Polymerase Chain Reaction Amplification Protocol for SequencingBased Typing of Human Leukocyte Antigen DRB1

    【Abstract】ObjectiveTo develop a new singletube polymerase chain reaction amplification (ST Amp) protocol for the efficient sequencing-based typing (SBT) of human leukocyte antigen DRB1(HLA-DRB1).MethodsA set of 7 group-specific exonic 5′ amplification primers and a single generic 3′ primer were included together in a single PCR mix to facilitate a single PCR amplification per sample for HLA-DRB1 typing.ResultsAll samples were successfully typed, the typing result was accurate and repeatable.ConclusionST Amp technique has resulted in the ability to perform high-resolution, high-specificity and high-throughput HLA-DRB1 typing by DNA sequencing.

    Release date:2016-09-08 11:54 Export PDF Favorites Scan
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