In order to investigate the therapeutic effects of ultrashort wave and He-Ne laser on experimentally infected skin flaps, 24 lower abdominal skin flaps on 24 rabbits were established, under each flap 1 ml of S. aureus (9 x 10(8) bacterials/ml) was injected respectively. Then, ultrashort wave and He-Ne laser were utilized respectively in 2 groups once a day for 6 days, with on treatment in two another two groups as control groups. After the period of treatment, the 4 groups were evaluated in the bacterial amounts, thickness of skin flaps and degree of infection. The results showed that the skin flaps in the treatment groups were much better than those in the control groups. It suggested that both the ultrashort wave and He-Ne laser are helpful in the treatment of infection of S. aureus in skin flaps.