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find Keyword "KLF2" 2 results
  • The relationship between KLF2/RelA imbalance and neutrophil apoptosis in asthmatic patients

    ObjectiveTo determine whether there is an imbalance of KLF2/RelA in peripheral blood neutrophils in patients with bronchial asthma, and explore the relationship between KLF2/RelA imbalance and neutrophil apoptosis.MethodsFrom April 2011 to April 2012, a total of 39 patients with acute attack of asthma in Hunan People's Hospital and Third People's Hospital of Changsha were enrolled, with 13 cases in mild asthma group, 17 cases in moderate asthma group, and 9 cases in severe asthma group. Fifteen healthy subjects were recruited as control group. Peripheral blood were collected from all subjects followed by separation of neutrophils. The apoptosis of neutrophils were measured by flow cytometry. The expression of KLF2 and RelA were detected by Western blot. The relationship between the ratio of KLF2/RelA and neutrophil apoptosis rate was analyzed by Pearson correlation test.ResultsNeutrophil apoptosis rates in the mild, moderate and severe asthma groups [(4.45±0.76)%, (2.10±0.25)%, (1.81±0.67)%, repectively] were lower than that in the healthy control group [(5.36±0.57)%, all P<0.01]. The apoptosis rates of neutrophils in the moderate and severe asthma groups were lower than that in the mild asthma group (bothP<0.01), but there was no significant difference between the moderate asthma group and the severe asthma group (P>0.05). The ratios of neutrophil KLF2/RelA in the mild, moderate and severe asthma groups were lower than that in the normal control group (0.667±0.351, 0.384±0.203, 0.536±0.293vs. 4.038±2.011, all P<0.01). There was no significant difference between the groups of mild, moderate and severe asthma (P>0.05). The neutrophil apoptosis rate was positively correlated with the percentage of neutrophil KLF2/RelA (r=0.592 0, P<0.000 1).ConclusionThere is an imbalance of KLF2/RelA in peripheral blood neutrophils in patients with bronchial asthma, and the imbalance of KLF2/RelA may be the mechanism of apoptosis of peripheral blood neutrophils.

    Release date:2018-01-23 01:47 Export PDF Favorites Scan
  • KLF2调控S1P1~S1P5对类中性粒细胞迁移的作用机制

    目的 研究KLF2是否通过调控S1P1~S1P5影响类中性粒细胞迁移。方法 构建携带有siRNA的慢病毒载体,特异性沉默KLF2基因,利用细胞迁移实验检测类中性粒细胞迁移情况,利用蛋白印迹法、实时PCR分别检测KLF2、S1P1~S1P5蛋白和mRNA表达情况。结果 ① KLF2病毒干扰组较空白组、空载病毒组,迁移率明显减少(P<0.05),S1P从0 µmol/L至0.1 µmol/L,随着浓度的增加,各组类中性粒细胞迁移率都明显增加(P<0.05),S1P从0.1 µmol/L至0.4 µmol/L ,随着浓度的增加,各组类中性粒细胞迁移率基本相同(P>0.05);② KLF2病毒干扰组与空白组、空载病毒组比较,S1P1蛋白及mRNA相对表达量减少(P<0.05);③ KLF2病毒干扰组与空白组、空载病毒组比较,S1P2 、S1P3、S1P4、S1P5 mRNA表达量无差异(P>0.05)。结论 S1P对类中性粒细胞迁移的最佳浓度为0.1 µmol/L; KLF2通过正调控S1P1表达,促进类中性粒细胞迁移; 在类中性粒细胞中KLF2不调控S1P2~S1P5表达。

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