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find Author "Kashiwagi Kenji" 1 results
  • Matrix metalloproteinase in human diabetic and non-diabetic vitreous

    Purpose To identify matrix metalloproteinase (MMP) in human vitreous samples of diabetic vitreoretinopathy (DR) and other ocular diseases (non-DR) and to probe the related factors of MMP expression. Methods Thirty-one diabetic and 17 non-diabetic vitreous samples (nine macular hole and eight epiretinal membrane patients) were examined. Samples were concentrated and subjected to substrate zymography to conduct a quantitative analysis of MMP-2,9 activity. The technology of Western blotting against anti-human MMP-2,9 was performed to identify MMP in vitreous samples. Results Vitreous samples both from DR patients and from non-DR patients showed a single band at the position of 72 kDa, correspondin g to MMP-2. Quantitative analysis revealed that diabetic vitreous showed higher MMP-2 activity than non-DR, although the difference was not significant.45.2% of DR patients showed MMP-9, but no expression in non-DR.Among DR samples, the positive ratio of MMP-9 in partial posterior vitreous detachment (PVD)(66.7%) was significantly higher than that of complete PVD (15.4%). Western blotting study confirmed the expression of MMP-2 and MMP-9. Conclusion There is no obvious difference of MMP-2 activity between DR and non-DR. MMP-9 may be involved in the pathogenesis of diabetic vitreor etinopathy and the deterioration of proliferative change. (Chin J Ocul Fundus Dis, 2001,17:195-197

    Release date:2016-09-02 06:03 Export PDF Favorites Scan
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