ObjectiveTo investigate the survival of liver cancer cells in hypotonic solution, and provide a theoretical basis for the clinical application of warm distilled water to kill free intraperitoneal liver cancer cells. MethodsThe hepatocellular carcinoma cell line Hep3B were cultured by using different osmotic HEPES buffered solution (148 mOsmol/kg, 90 mOsmol/kg, and 0 mOsmol/kg) in vitro, and then the activity and status of the cells were observed by using the MTT assay and flow cytometry. ResultsOne hundred and forty-eight mOsmol/kg and 90 mOsmol/kg osmotic pressure solution had no obvious killing effect for hepatocellular carcinoma cell line Hep3B, the cells still had strong activity after 30 min. The 0 mOsmol/kg osmotic pressure distilled water had particular killing effect for hepatocellular carcinoma cells, with the prolongation of time and the killing effect was enhanced. It needs 10 min to destruct free cancer cells. ConclusionApplication of warm distilled water lavage in liver cancer surgery requires the continuing role of 10 min to kill the intraperitoneal free cancer cells, thereby preventing the occurrence of postoperative peritoneal metastasis.
ObjectiveTo summarize the recent development of the magnetic compression anastomosis in surgery. MethodsThe abroad and domestic relevant literature about magnetic compression anastomosis was extensively reviewed, and comprehensive analyzed. ResultsMagnetic compression anastomosis in the reconstruction of the body lumen is a simple and effective way; laparoscopy combined with magnetic compression anastomosis has the advantages of small wound and quick recovery. Magnetic compression anastomosis has been increasingly used in cl inical practice. But its mechanism has not been fully revealed, and it is difficulty in expelling out of the magnet for the gastrointestinal and certain large-size lumen, so further research and optimization are still needed. ConclusionRelative to the traditional manual suture, magnetic anastomosis has great advantage, especially a combination of magnetic compression anastomosis and minimally invasive surgery has bright prospects for development.
ObjectiveTo investigate the advantages of magnetic compressive anastomosis (MCA) for non-suture femoral artery anastomosis. MethodsTwelve adult health mongrel dogs,weighing (16.5±3.6) kg,were selected for in situ end-to-end anastomosis of the femoral artery.One side of the femoral artery was anastomosised with MCA (group A) and the other side of the femoral artery was anastomosised by hand-suturing (group B).The anastomosis time,complications,and vascular bursting pressure were recorded.Gross observation,histological staining (HE and Masson),and scanning electron microscopy observation were performed at 2,4,12,and 24 weeks postoperatively. ResultsThe anastomosis time in group A [(3.89±1.16) minutes] was significantly shorter than that in group B [(14.16±3.72) minutes] (t=14.226,P=0.000).The complication rate of group A (0) was significantly lower than that of group B (75%)(P=0.000).At immediate,4 and 12 weeks after operation,the vascular bursting pressure of anastomosis site in group A was more than 280 mm Hg (1 mm Hg=0.133 kPa),and was (140.11±15.23),(180.31±24.55),and more than 280 mm Hg in group B,showing significant differences at immediate and 4 weeks (P<0.05),but no significant difference at 12 weeks (P>0.05).In group A at 4 weeks,good intima contact,smooth endothelium,and regular arrangement of endothelial cells were observed;at 12 weeks,chronic inflammation was present,with a few lymphocytes infiltration;and at 24 weeks,inflammation significantly decreased.But in group B,obvious suture foreign body and scar formation were observed,which led to uneven surface with lumen incomplete intima,and irregular endothelial cells in arrangement disorder. ConclusionCompared with traditional hand-suturing,the MCA has the advantages of shorter operation time,higher patency rate,less complication,and better healing at the anastomotic site.Non-suture anastomosis of the femoral artery by MCA can achieve reliable results.