Objective To detect the expressions of RhoA and Snail in gastric cancer tissues， and explore the relati-onship of these expressions to the biological behavior of the gastric cancer. Methods The expressions of RhoA and Snail protein in the paraffin-embedded specimens of 189 gastric cancer patients were detected by immunohistochemical method. The relationships of their expressions to clinicopathologic features of gastric cancer or survival， and the relevance of RhoA expression and Snail expression were analyzed. Results ① The expressions of RhoA and Snail protein in the gastric cancer tissues were significantly higher than those in the paraneoplastic tissue （RhoA：P=0.008；Snail：P=0.000） and the normal gastric mucosa tissue （RhoA：P=0.010；Snail：P=0.000）；The expression of RhoA had no significant difference between the paraneoplastic tissue and the normal gastric mucosa tissue （P=0.782）， however， the expression of Snail in the paraneoplastic tissue was significantly higher than that in the normal gastric mucosa tissue （P=0.001）. ② The expression of RhoA in the gastric cancer tissue was associated with TNM staging and Lauren type （P＜0.05）， but it was not associated with tumor diameter， lymph node metastasis， or differentiation degree （P＞0.05）. The expression of Snail in the gastric cancer tissue was associated with tumor diameter， lymph node metastasis， differentiation degree， TNM staging， or Lauren type （P＜0.05）. The expressions of RhoA and Snail in the gastric cancer tissue were not associated with patients’ gender and age （P＞0.05）. ③ The expression of RhoA protein was significantly positi-vely correlated with Snail protein in the gastric cancer（rs=0.203， P=0.005）. ④ The TNM staging of tumor， RhoA and Snail expressions， and lymph node metastasis were all the independent prognostic factors of postoperative gastric cancer patients （P＜0.05）. Conclusions RhoA and Snail proteins express in gastric cancer tissues， and involves in gastric carcinogenesis and the development process， and RhoA/Snail signaling pathway may play an important role in invasion and metastasis of gastric cancer.