Selective recognition of double strands DNA (dsDNA) has been a research hot spot in molecular biology and biomedicine for a couple decades. Based on the selective interaction between natural nucleic acid/synthetic molecular ligands and dsDNA, gene diagnosis, gene therapy and gene editing would be realized. Hairpin oligopolyamide is a molecular ligand with excellent cellular permeability and nucleases-resistance which can target dsDNA sequence with high affinity and specificity at minor groove. This paper reviews the binding properties and biomedical applications of hairpin oligopolyamide targeting dsDNA, which provide references for further design and application of hairpin oligopolyamide.
ObjectivesTo investigate the occurrence and parents’ cognition of accidental injury among pre-school children in Nanchong city, and provide advice and countermeasures to reduce accidental child injuries.MethodsUsing the multi-stage cluster sampling method, a total of 945 students and parents from 3 classes in each of 4 kindergartens in three districts of Nanchong city were surveyed with questionnaire.ResultsA total of 945 questionnaires were issued and 858 valid questionnaires were returned and the effective response rate was 90.79%. The incidence of incidental injury of pre-school children in Nanchong city was 25.99%, with no difference between boys and girls, and no difference between age groups. The top three injuries were falling (35.64%), smashing/touching/squeezing (22.11%), cutting/stabbing (10.56%). The child smashing/touching/squeezing rate was higher in boys than in girls, the difference was statistically significant (χ2=5.549, P=0.018). The top three places of the occurred injury were outdoor (53.67%), home (43.58%), and playground and kindergarten (13.76%). Between parents who possess injury-related knowledge and those who didn’t, there was no difference in the incidence of accidental injuries among their children. Parents often learned from cell phone (23.88%), TV (21.76%) and computer (18.11%). Ways in which they most hoped to learn from were the school advertised education (22.67%), TV (18.80%) and cell phones (17.75%). Of all types of emergency management skills, the top three figures acquired by the largest population were post-burn emergency treatment (72.03%), emergency treatment for traumatic bleeding (52.56%) and emergency treatment of animal bites (37.53%).ConclusionsThe incidence of accident injuries is high in urban areas of Nanchong city. The safety management of home and kindergarten should be strengthened, including schools' safety education and skills training for children and parents.
Objective To investigate the organizational structure, routine management, emergency response, and material reserves related to the prevention and control of infectious diseases in maternal and child health institutions at all levels in Sichuan, and to understand the construction of emerging infectious diseases prevention and control system in maternal and child health institutions throughout the province. Methods We conducted a survey on the current situation of the epidemic prevention and control system in maternal and child health institutions using a self-developed questionnaire, which was conducted in October 2020 and July 2021, respectively. We conducted comparative analysis on the basic situation, the construction of emerging infectious disease prevention and control systems, and the setting and management of fever clinics/rooms of maternal and child health institutions at all levels in Sichuan in 2020 and 2021. Results In 2020, a total of 166 maternal and child health institutions participated in the survey, and 166 questionnaires were collected, including 17 at the provincial and municipal levels and 149 at the county level. In 2021, a total of 182 maternal and child health institutions participated in the survey, and 182 questionnaires were collected, including 17 at the provincial and municipal levels and 165 at the county level. In 2021, all levels of maternal and child health institutions in Sichuan had established epidemic prevention and control leadership groups. Compared with 2020, the construction of the emerging infectious disease prevention and control system in maternal and child health institutions at all levels in Sichuan in 2021 had improved to a certain extent in terms of establishing epidemic prevention and control leadership groups, hospital area three channel management, three-level pre-examination triage, inpatient area allocation, staff management, and patient management (P<0.05). Compared with 2020, fever clinics that met the requirements of three zones and two channels management, fever clinics that met the requirements of closed-loop management, and management of fever clinics/rooms in maternal and child health institutions at all levels in Sichuan in 2021 had improved to a certain extent (P<0.05). Conclusion Through strengthened construction during the epidemic prevention and control period, the construction of the emerging infectious disease prevention and control system in maternal and child health institutions in Sichuan has improved, but still faces continuous challenges in normalized prevention and control.