ObjectiveTo explore the dyeing conditions of reticular fiber staining of liver puncture tissue stained by BenchMark automatic special staining instrument, evaluate the staining effect, and share the using experience.MethodsA total of 30 cases of liver puncture specimens from April to May 2019 in West China Hospital of Sichuan University were selected. They were fixed with 4% neutral formaldehyde fix solution, treated with automatic dehydrator, embedded in paraffin, stained with adhesive slides, and baked in 65℃ oven for 30 min. Each specimen was sliced in duplicate, with a thickness of 5 μm. One was reticular fiber stained with BenchMark automatic special staining instrument under the dyeing condition according to the using experience of the laboratory, and the other was reticular fiber stained by manual Foot staining method according to the standard operating procedure of the laboratory. The staining effect under microscope was observed by a chief pathologist with extensive slide-reading experience.ResultsThe success rate of instrumental method was 100.0% (30/30), and that of the manual method was 76.7% (23/30). The difference in success rate between the two methods was statistically significant (χ2=5.143, P=0.023).ConclusionThe reticular fiber staining effect of BenchMark automatic special staining instrument is stable and reliable, and the success rate is higher than that of manual Foot staining.
Quality control refers to the general term of operation technology which activities to meet the quality requirements. Quality control is not only the need of the development of the laboratory itself, but also the important requirement of the laboratory accreditation organization for the development of the laboratory. To carry out quality control, first of all, a complete quality control system should be established. According to the pathological experiment process, which can be divided into pre-analysis, in-analysis and post-analysis quality control. There are some similarities between special staining and hematoxylin-eosin staining, but the details are slightly different. There are many kinds of special dyeing items, and each dyeing step is complicated, so it is difficult to control the quality according to the quality control technology of conventional pathological staining. This article reviews the whole process quality control of special dyeing by summarizing the literature and work experience, so as to contribute to the subsequent improvement of the quality of special dyeing.
Objective To establish the reference intervals for platelet-related parameters among the elderly population in Longquanyi District, Chengdu. Methods From November 1st to December 31st 2021, elderly individuals aged between 60 and 80 years were selected from the Longquanyi branch of a natural population cohort study initiated by West China Hospital of Sichuan University in 2021. Complete blood cell analysis was performed to obtain the platelet-related parameters. IBM SPSS Statistics 25 software was used to analyze the data and establish the reference intervals for platelet-related parameters among the elderly population in Longquanyi District. Results A total of 1938 reference individuals were selected by inclusion and exclusion criteria, and finally 1669 reference individuals, including 665 males and 1004 females, were included to establish the reference intervals after excluding outliers. The reference range of blood platelet count was (95.03-301.32)×109/L for elderly men and (112.13-320.75)×109/L for elderly women, respectively. The reference range of mean platelet volume was 8.97-13.80 fL for elderly men and 9.10-13.90 fL for elderly women, respectively. The reference range of plateletcrit was 0.13%-0.30% for elderly men and 0.14%-0.32% for elderly women, respectively. The reference range of platelet distribution width was 15.80-16.90 for elderly men and 15.70-16.90 for elderly women, respectively. Conclusion It is of practical significance to establish platelet-related parameters in the elderly in local region.