Objective To prepare the immunonanospheres［SC3Ab-HSA(5-Fu)-NS］ against human colorectal cancer and evaluate its immunoreactivity and effects on cancer. Methods SC3Ab-HSA(5-Fu)-NS was prepared by intermolecular cross-linking the monoclonal antibody SC3Ab with human serum albumin nanospheres containing 5-Fu ［HAS(5-Fu)-NS］ via new hetero-bifunctional crosslinker SPDP. Condensation test and immunoflurecence were used to evaluate the immunoreactivity, the specific binding of SC3Ab-HSA(5-Fu)-NS with colorectal cancer cell line SW1116 was observed by microscope and electron microscope. The specific cytotoxic effects on target cells were evaluated in vitro by MTT assay. SC3AbHSA(5-Fu)-NS, HSA(5-Fu)-NS and 5-Fu were injected into nude mice bearing human colorectal carcinoma, to study the inhibitory activity of SC3Ab-HSA(5-Fu)-NS in vivo. Results The immunoreactivity of SC3Ab-HSA(5-Fu)-NS was well preserved. SC3Ab-HSA(5-Fu)-NS can bind the SW1116 cells specifically. The IC50 value for SC3Ab-HSA(5-Fu)-NS on SW1116 cells was 24.6 μg/ml,which was lower than that of HSA(5-Fu)-NS(345.3 μg/ml) and 5-Fu(325.6 μg/ml). The inhibitory rate of SC3Ab-HSA(5-Fu)-NS on the growth of colorectal cancer xenografts was significantly higher than that of HSA(5-Fu)-NS or 5-Fu(P＜0.001).Conclusion SC3Ab-HSA(5-Fu)-NS has immunoreactivity and specific active targeting to the colorectal cancer cells. The anticancer ability of SC3Ab-HSA(5-Fu)-NS is significantly higher than that of HSA(5-Fu)-NS and 5-Fu.
ObjectivesTo systematically review the detection rate of depression in Chinese individuals with type 2 diabetes mellitus (T2DM).MethodsPubMed, EMbase, The Cochrane Library, CBM, CNKI, WanFang Data and VIP databases were electronically searched to collect observational studies on the detection rate of depression in Chinese with T2DM from inception to January, 2019. Two reviewers independently screened literature, extracted data and evaluated the quality of included studies. Meta-analysis was performed by R 3.6.1 software.ResultsA total of 29 studies involving 96 557 cases were included. Meta-analysis results showed that the total detection rate of depression in Chinese with T2DM was 27% (95%CI 24% to 30%). Subgroup analysis showed that the detection rate of depression in female was 32% (95%CI 20% to 45%), and in male was 26% (95%CI 18% to 37%). The detection rate of depression in rural areas was 36% (95%CI 18% to 54%), and in urban areas was 30% (95%CI 16% to 43%). The detection rate of depression in individuals aged 60 and above was 31% (95%CI 24% to 39%), and in individuals aged less than 60 was 23% (95%CI 10% to 36%). Individuals with a primary school education and below, a middle or high school education and college degree and above education had detection rate of 31%, 23% and 22%, respectively. Individuals with diabetes duration less than 5 years, 5 to 10 years and more than 10 years had detection rates of 23%, 25% and 30%, respectively. Individuals with and without complications had detection rates of 43% and 26%, respectively. The detection rates of mild and moderate to major depression were 20% and 10%, respectively.ConclusionsThe detection rate of depression in Chinese with T2DM is high. Due to limited quality and quantity of the included studies, more high quality studies are required to verify above conclusion.
ObjectiveTo systematically review the detection rate of cognitive impairment in Chinese patients with type 2 diabetes mellitus (T2DM).MethodsPubMed, EMbase, The Cochrane Library, CBM, CNKI, WanFang Data and VIP databases were searched to collect studies on the detection rate of cognitive impairment in Chinese patients with T2DM from inception to January 20th, 2021. Two reviewers independently screened literature, extracted data and evaluated the risk of bias of included studies. Meta-analysis was then performed using Stata 12.0 software.ResultsA total of 27 studies involving 7 920 cases were included. Meta-analysis results showed that the total detection rate of cognitive impairment in Chinese patients with T2DM was 43.2% (95%CI 36.9% to 49.6%). The results of subgroup analysis showed that in T2DM patients, the detection rate of cognitive impairment in males was 42.4% (95%CI 34.4% to 50.4%), and that in females was 48.2% (95%CI 40.9% to 55.6%). The detection rate of cognitive impairment was 25.4% (95%CI 14.7% to 36.0%) in patients under the age of 60 years, and 47.0% (95%CI 30.0% to 64.0%) in patients aged 60 years or above. The detection rate of cognitive impairment among those with primary school education level or below was 67.1% (95%CI 48.9% to 85.3%). The detection rate of cognitive impairment was 37.1% (95%CI 27.3% to 46.8%) among those with education level of junior high school or above. The detection rate of cognitive impairment in patients with disease duration less than 10 years was 28.4% (95%CI 16.0% to 40.9%) and that in patients with disease duration more than 10 years was 50.6% (95%CI 33.2% to 68.0%). The detection rate of cognitive impairment in married individuals was 45.6% (95%CI 35.8% to 55.4%) and that in singles was 68.1% (95%CI 57.5% to 78.7%). The detection rate of cognitive impairment in smokers was 38.9% (95%CI 30.7% to 47.2%) and in non-smokers was 40.9% (95%CI 32.1% to 49.6%). The detection rate of cognitive impairment in drinkers was 35.6% (95%CI 27.3% to 44.0%) and that in non-drinkers was 41.8% (95%CI 32.2% to 51.4%).ConclusionsThe detection rate of cognitive impairment in Chinese patients with T2DM is high. Due to the quantity and quality of included studies, more high-quality studies are needed to verify the above conclusions.
ObjectiveTo systematically review the diagnostic accuracy of MRI in preschool children with autism spectrum disorder. MethodsDatabases including Web of Science, PubMed, The Cochrane Library, EMbase, CBM, CNKI, WanFang Data, and VIP were electronically searched for studies on MRI in diagnosis of preschool children with autism spectrum disorder from inception to January 2022. Two reviewers independently screened the literature, extracted data, and assessed the risk of bias the included studies. Then, meta-analysis was performed using Meta-Disc, RevMan 5.4 and Stata 16.0 software. ResultsA total of 17 studies were finally included. The pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnosis odds ratio and area under the curve of SROC were 0.78 (95%CI 0.76 to 0.81), 0.77 (95%CI 0.75 to 0.79), 3.92 (95%CI 2.83 to 5.41), 0.28 (95%CI 0.23 to 0.35), 14.8 (95%CI 9.31 to 23.52) and 0.86, respectively. Subgroup analysis revealed that the source of patients, age, prediction methods and analysis features were potential sources of heterogeneity. Heterogeneity in the subgroup of 1-3 years old was small, and the pooled sensitivity, specificity and area under the curve of SROC were 0.81 (95%CI 0.76 to 0.85), 0.82 (95%CI 0.78 to 0.86) and 0.87, respectively. The pooled sensitivity, specificity and area under the curve of SROC for non-site studies were 0.80 (95%CI 0.75 to 0.84), 0.80 (95%CI 0.76 to 0.85) and 0.86, respectively. ConclusionMRI has a high accuracy in the diagnosis of preschool children with autism spectrum disorder; however, the possibility of misdiagnosis and missed diagnosis should be considered. Due to the limited quantity and quality of the included studies, more high-quality studies are needed to verify the above conclusion.
ObjectiveTo analyze the quality of the randomized controlled trials (RCTs) included in the systematic review in the anti-infection field in the elderly.MethodsA comprehensive and systematic literature search in PubMed, EMbase, CNKI, The Cochrane Library, WanFang Data, VIP and CBM was conducted to collect systematic review or meta-analysis which involoved anti-infection RCTs in the elderly from inception to February 17th, 2020. The results of Cochrane risk of bias assessment of the included RCTs were analyzed.ResultsA total of 8 systematic reviews were included, involving 19 RCTs and 6 735 participants. The sample size of the RCTs ranged from 23 to 2538, and the published date were from 1980 to 2020. The included RCTs focused on postoperative infection, urinary tract infection, Clostridium Difficile infection and so on. The included RCTs had methodological quality issues. Among the assessment results of low risk of bias, the domains of selection bias (random sequence generation) and selection bias (allocation hiding) had the lowest proportion (47.3%, 36.8%). Among the assessment results of unclear risk of bias, the domains of selection bias (random sequence generation) and selection bias (allocation hiding) had the highest proportion (42.1%, 52.6%). Among the assessment results of high risk of bias, the domains of measurement bias and performance bias had the highest proportion (21.1%, 21.1%).ConclusionsThe quality of RCTs in the field of anti-infection in the elderly requires further improvement. High-quality anti-infection RCTs for the elderly should be developed in future to better guide clinical practice.