ObjectiveTo evaluate the relation between protein expression of RAS-association domain family 1A (RASSF1A) gene and the occurrence and development of vulvar cancer. MethodsImmunohistochemistry was used to detect the expression of RASSF1A protein in 9 normal vulvar tissues, 12 vulvar intraepithelial neoplasm (VIN) tissues, as well as 35 vulvar cancer tissue samples which were taken from patients treated during January 2008 to December 2010. Then, we analyzed the correlation between the RASSF1A protein expression and the clinical pathological features of vulvar cancer. ResultsThe expression of RASSF1A protein in vulvar cancer samples was significantly different from that in samples of normal vulvar tissues and VIN tissues (P<0.05). Significant difference also existed in the expression of RASSF1A protein between normal vulvar tissues and VIN tissues (P<0.05). The expression of RASSF1A protein was not significantly correlated with age, grade, stage, lymph nodes involvement and sites (lateral/median) of vulvar cancer (P>0.05). ConclusionInactivation of RASSF1A gene involves in the occurrence of vulvar cancer but has no significant correlation with its development. There is no obvious correlation between cancer sites, onset ages and the expression of RASSF1A protein.
ObjectiveTo summarize the short-term effectiveness of TightRope system for the treatment of syndesmotic tibiofibular diastasis. MethodsBetween June 2013 and June 2015, 23 patients with syndesmotic tibiofibular diastasis were treated by TightRope system, including 15 males and 8 females with an average age of 32.3 years (range, 16-63 years). Injury was caused by sports in 13 cases, by traffic accident in 6 cases, and by falling from height in 4 cases. The locations were the left side in 13 cases and the right side in 10 cases. The time from injury to operation was 6 hours to 12 days (mean, 3.8 days). According to Lauge-Hansen classification, 13 cases were rated as supination-external rotation type, 7 cases as pronation-abduction type, and 3 cases as pronation-external rotation type; according to Weber classification, 5 cases were rated as type A, 11 cases as type B, and 7 cases as type C. ResultsThe mean operative time was 68.1 minutes (range, 48-93 minutes); the mean intraoperative blood loss was 70.3 mL (range, 20-150 mL); and the mean hospitalization days were 7 days (range, 5-13 days). Superficial local skin necrosis occurred in 6 cases, and primary healing of incision was obtained in the others. All patients were followed up 8-30 months (mean, 16.4 months). X-ray films showed bone union was achieved within 6-12 weeks (mean, 9.4 weeks). No related complications of reduction failure and re-fracture occurred. The internal fixators were removed at 10-18 months postoperatively (mean, 13.3 months). According to American Orthopaedic Foot and Ankle Society (AOFAS) score for ankle function evaluation, the results were excellent in 19 cases and good in 4 cases at last follow-up. ConclusionTightRope system is a good method to treat syndesmotic tibiofibular diastasis, because of safety, convenient operation, and satisfactory short-term effectiveness.
ObjectiveTo systematically review the effectiveness and safety of combined spinal-epidural anesthesia for cesarean section. MethodsWe searched The Cochrane Library (Issue 10, 2013), PubMed, MEDLINE, EMbase, CBM, CNKI, VIP and WanFang Data for randomized controlled trials on combined spinal-epidural anesthesia for cesarean section up to October 2013. Two reviewers independently screened literature according to the inclusion and exclusion criteria, extracted data, and assessed the methodological quality of included studies. Then meta-analysis was conducted using RevMan 5.2.9 software. ResultsA total of 9 studies containing 616 delivery women were included. The results of meta-analysis showed that:compared with epidural anesthesia, combined spinal-epidural anesthesia was superior in the time of sensory blockade to T4 (MD=-7.38, 95%CI-9.54 to-5.23, P < 0.000 01), muscle relaxation (OR=6.09, 95%CI 2.65 to 13.97, P < 0.000 1), and recovery of motor block (MD=-41.57, 95%CI-58.98 to-24.17, P < 0.000 01). Compare with spinal anesthesia, combined spinal-epidural anesthesia was superior in lowering the incidence of low blood pressure (OR=0.49, 95%CI 0.29 to 0.81, P=0.006). ConclusionCurrent evidence shows that combined spinal-epidural anesthesia could increase the effectiveness and safety of combined spinal-epidural anesthesia for cesarean section.
ObjectiveTo analyze impact of body mass index (BMI) on postoperative complications and disease-free survival (DFS) after hepatectomy for patients with hepatocellular carcinoma (HCC). MethodsIn total, 858 patients with HCC underwent hepatectomy were analyzed by retrospective cohort study. Patients were divided into two groups according to BMI:normal group (18.5 kg/m2 < BMI < 25.0 kg/m2) and obesity group (BMI≥25.0 kg/m2). The clinical and postoperative follow-up data were collected and statistically analyzed. Results① Compared with the normal group, the preoperative HBV-DNA loading was significantly lower (P<0.05), albumin was significantly higher (P<0.05), intraopera-tive blood loss was significantly increased (P<0.05), operation time, and the first portal hepatis occlusion time were signifi-cantly prolonged (P<0.05) in the obesity group. The postoperative complications and hospital stay had no significant differences between these two groups (P>0.05). ② The results of univariate analysis showed that the preoperative HBV-DNA≥ 104 U/mL, total bilirubin >21 μmol/L, albumin <35 g/L, grade B of Child-Pugh, intraoperative blood loss >500 mL, and operation time >240 min were associated with the postoperative complications after hepatectomy for patients with HCC (P<0.05). The results of multivariate analysis showed that preoperative total bilirubin >21 μmol/L, albumin <35 g/L, and operation time >240 min were the independent risk factors for postoperative complications (P<0.05). ③ Kaplan-Meier analysis showed that the 3-year DFS in the obesity group was significantly better than that in the normal group (P<0.05). The results of multivariate analysis showed that the major blood vessel tumor thrombi, multicenter tumor, tumor diameter ≥5 cm, and operation time >240 min were the independent risk factors for DFS (P<0.05), while the obesity was the protective factor for DFS (P<0.05). ConclusionFor HCC patients who receiving hepatectomy, obesity does not increase risk of postoperative complications, and could increase 3-year DFS. Thus preoperative improvement of nutritional status of patient with HCC has a great significance.
ObjectiveTo investigate the clinical efficacy of catheter directed thrombolysis (CDT) through three different approaches combined with iliac venous endovascular therapy for acute deep venous thrombosis (DVT) complicated with Cockett syndrome of the lower extremities. MethodThe clinical data of 87 patients with CDT through three different approaches (small saphenous vein group, popliteal vein group, and posterior tibial vein group) combined with iliac venous endovascular therapy for DVT complicated with Cockett syndrome of the lower extremities were analyzed retrospectively. ResultsThe lower extremity swelling of all the patients were disappeared obviously within 72 h after surgery, there was no death related surgery and pulmonary embolism. The limb edema reduction rates had no significant differences among the small saphenous vein group, popliteal vein group, and posterior tibial vein group﹝(77±13)% versus (82±12)% versus (77±18)%, P > 0.05﹞. The recanalization rates of thrombolysis had no significant differences among the above three groups﹝(86.5±10.6)% versus (92.0±7.7)% versus (87.3±7.8)%, P > 0.05﹞. The time required for the cannulation in the posterior tibial vein group was significantly shorter than that of the small saphenous vein group or popliteal vein group﹝(15.14±3.62) min versus (32.62±9.36) min or (42.79±13.30) min, P < 0.01﹞. All the patients were performed by balloon dilatation and iliac vein stenting. Eighty-seven cases were followed-up for 1-24 months, the primary patency rate of iliac venous was 100%. ConclusionsCDT with iliac venous endovascular therapy is an effective method in treatment of acute DVT with Cockett syndrome. CDT through posterior tibial vein is an easier and effective method with less complications and time. This way could be acceptable in basal hospital.
ObjectiveTo explore clinical value of 3D printing technology in hepatic resection. MethodsFrom March to May 2015, multidetector-row computed tomography images of 12 patients, including hepatic carcinoma in 6, hepatic hemangioma in 3, intra-and extra-hepatic bile duct stones in 3, were used for 3D hepatic reconstruction, the final segmentation data were converted to stereolithography files for 3D printing, 50%-70% scale of the full-sized liver model was fabricated by polylactic acid to be used to analyze its anatomical structure, design surgical planning, select the optimal operative route and simulate hepatic resection. Hepatic resection was performed by referring to the 3D printing model. ResultsThe hepatic resections were successful without complications by referring to the preoperative 3D printing models, the average blood loss was 340(100-1000) mL. ConclusionHepatic resection is more accurate and safe by 3D printing technology.