ObjectiveTo explore the effect of the serum high mobility group box-1 (HMGB1) on oncosis of pancreatic acinar cells in the rat with severe acute pancreatitis (SAP). MethodsThirty-two healthy SD rats were randomly divided into 2 groups:sham operation group (SO group, n=8) and SAP group (n=24). Rats of SO group were only flipped the intestinal canal after laparotomy, but rats of SAP group were induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in addition. Rats of SO group were sacrificed at 6 hours after operation, and rats of SAP group were sacrificed at 6 (SAP-6 hour group, n=8), 12 (SAP-12 hour group, n=8), and 24 hours (SAP-24 hour group, n=8) after operation respectively. Pancreatic tissues were stained by HE to observe pathological changes. Serum HMGB1 was measured by ELISA, and the oncosis percentage of pancreatic acinar cells was examined by flowcytometry. ResultsPathological results showed that structural integrity was observed in pancreatic acinar, and occasionally a single inflammatory cell infiltration was observed in rats of SO group. Swelling, interstitial edema, and inflammatory cell infiltration were observed in rats of SAP-6 hour group. Some necrosis of pancreatic acinar cell, stromal vascular congestion, and focal necrosis were observed in rats of SAP-12 hour group and SAP-24 hour group, which the pathological damage were worse over time. Levels of serum HMGB1 and oncosis percentages of pancreatic acinar cells in rats of 3 SAP subgroups were all higher than those of SO group (P < 0.01), and the 2 kinds of indexes both increased over time (P < 0.05). There was positive correlation between concentration of serum HMGB1 and oncosis percentages of pancreatic acinar cells in SAP rat during 24 hours after operation (r=0.846, P < 0.01). ConclusionsHMGB1 seems to play an important role in SAP by inducing oncosis of pancreatic acinar cells when inducing inflammatory reaction in rat with SAP.
ObjectiveTo observe the clinical efficacy and safety of reteplase in prehospital thrombolysis for ST-segment elevation acute myocardial infarction. MethodsWe retrospectively analyzed the clinical data of 62 patients with acute ST-segment elevation myocardial infarction treated in our hospital between September 2010 and December 2012.They were randomly divided into two groups:the treatment group with 22 patients given reteplase thrombolysis therapy in the prehospital ambulance and/or emergency department,and the control group with 40 patients receiving thrombolytic therapy in the hospital.Then,we compared 60-minute and 120-minute recanalization rate,the rate of complicating with various kinds of adverse reactions and the composite end-point event rate between the two groups. ResultsSixty minutes and 120 minutes after thrombolysis,the clinical judgment recanalization rate in the treatment group was significantly higher than that in the control group (P<0.05).Four weeks after hospitalization,the rate of complicating with various kinds of hemorrhage,hypotension and death rate in the two groups had no statistical difference (P>0.05). ConclusionPrehospital thrombolysis treatment for ST-segment elevation acute myocardial infarction has a better clinical efficacy and is worth popularizing in basic unit hospitals.
ObjectiveTo explore the validity period of oral sterilizing instruments in different packages inside the bio-safety cabinet. MethodsInside the bio-safety cabinet, we brought the autoclaved dental gadgets packed in glassware and paper-plastic into microbial sampling cultivation experiment, and made observation of the bacteria growth at the 4th, 8th, 24th hour after sterilizing, with the sampling of once per 24 hours, lasting 10 days. Samples taken from the gadgets in paper-plastic each time when samples were taken from the gadgets in glassware were as the control. ResultsUnder the condition that the bio-safety cabinet was not contaminated, no colony grew in neither group in 144 hours; 168 hours later, the cultivation results indicated that colonies started to grow（P<0.05）. ConclusionUnder the condition that the bio-safety cabinet is pollution-free, the validity period of gadgets in glassware packaging for dental clinic practices is far longer than the 4-hour limit after the sterilized packages are opened, as is stipulated in the "Operating Specifications for Sterilization Technology on Dental Instruments of Medical Institutions".
ObjectiveTo investigate the value of morning blood pressure surge in the evaluation of atherosclerosis by the observation of the relationship between morning blood pressure surge and ambulatory arterial stiffness index. MethodsFrom May 2011 to March 2013, we followed up 300 hypertensive patients who received ambulatory blood pressure monitoring, and they were divide into elevated morning blood pressure surge group and non-elevated morning blood pressure surge group. The AASI value was recorded and compared between the two groups according to the grade of hypertension. ResultsMorning blood pressure surge was more common in patients with hypertension. Compared with patients in the non-elevated morning blood pressure surge group, ambulatory arterial stiffness index was significantly higher in patients in the elevated morning blood pressure surge group. ConclusionThere is a certain correlation between morning blood pressure surge and arterial stiffness, which is an important factor leading to a variety of target organ damage.
Finger tapping test is a common testing item for patients with Parkinson's disease (PD) in clinical neurology. It mainly evaluates the fine motor function of patient's hand in three aspects:amplitude, speed and regularity of the movement. This paper focused on the quantitative assessment of regularity of finger tapping movement for PD patients. The movement signals of thumb and index finger were recorded by using inertial sensor unit in the process of tapping test. Two nonlinear dynamic indexes, approximate entropy (ApEn) and sample entropy (SampEn), were calculated, and then the values were statistically analyzed. The experimental results indicated that both indexes had significant differences between patient group and control group. Moreover, the indexes had relatively high correlation with the scores of corresponding unified Parkinson's disease rating scale (UPDRS) item rated by clinical clinician, which illustrated that these two indexes could reflect the injury level of the repetitive finger movement. So, as a reliable method, it can be provided to the clinical evaluation of hand movement function for PD patients.