ObjectiveTo construct an evaluation index system for the dissemination influence of new media health science popularization, providing a quantitative evaluation tool for comprehensively assessing the dissemination influence of health science communication works. MethodsMeta integration, Delphi method and analytic hierarchy process (AHP) were used to build a preliminary evaluation index system, form a framework of the evaluation index system, and calculate the weight values of the evaluation indexes, respectively. ResultsA total of 18 studies were included through retrieval. On the basis of preliminary evaluation index system, ten experts in related professional fields were invited to participate in two rounds of expert consultation, resulting in an evaluation index system framework that includes 2 first-level indicators, 4 second-level indicators, and 9 third-level indicators. ConclusionA preliminary evaluation index system for the dissemination and influence of new media health science communication works, which is reliable, universally applicable, and has a reasonable structure, is constructed.