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高功率微波对视网膜神经节细胞脂质过氧化作用的实验研究

Objective To determine the lipid peroxide damage in the primary cultured rabbit retinal ganglion cells induced by microwave． Methods Cultured rabbit retinal ganglion cells in vitro and exposed to 80 mW/cm2 of microwave for 15,30,45 min tespectively.Immediately after radiation,the morphological variation of cells was observed by optical microscope and transmission electronic microscope.Secondly,the activity of intracellular superoxide dismutase (SOD) and the content of malondialdehyde (MDA) were detected. Results Aportion of cells congregated,with their axon disapeared after radiation.Mitochondria and endoplasmic reticulum revealed swelling under transmission electronic microscope.The content of MDA was increased obviously compared with control group while SOD decreased．The content of MDA as increased obviously compared with control group after 45 min radiation was 5.11 times,while SOD decreased.The content of MDA as in control and the ganglion cells were apparantly destroyed. Conclusion Microwave can induce the lipid peroxide damage in primary cultured retinal ganglion cells,and lipid peroxide effect might be one of the mechanisms of microwave retinal damage. (Chin J Ocul Fundus Dis, 2000，16：32-34)