Objective To develop a reliable method for primary culture of normal human peritoneal mesothelial cells. Methods Human peritoneal mesothelial cells were dissociated by a mixture of pancreatin and ethylene diamine tetraacetic acid with a magnetic puddler. Inverted phase contrast microscope was used to observe the morphological structures of cells, approximate process of growth. Calretinin was used to identify the mesothelial cells. Results On the 4th d of culture, mesothelial cells adhered to the culture dish. After day 14, mesothelial cells confluenced gradually and grew well like the slabstone. Calretinin was positively expressed by mesothelial cells after 5 d of cultivation. The mesothelial cell population of subculture was less than that of the primary culture. Conclusion A reliable method for primary culture of normal human peritoneal mesothelial cells has been successfully developed, by which sufficient amount of highly purified normal human peritoneal mesothelial cells can be obtained.
Objective To find and evaluate the existence of distant peritoneal micrometastasis of gastric cancer in rectovesical pouch or Douglas pouch by using immunohistochemist ry method. Methods Forty cases of gastric cancer were collected f rom June 2004 to March 2006 in Nanjing Gulou hospital . None of them showed obvious distant peritoneal metastasis in preoperative physical and imaging examinations and laparotomy inspection or palpation. Tissues were taken f rom rectovesical pouch or Douglas pouch during the operations , and HE and CEA/ CK220 immunohistochemistry staining were then performed on the tissues. Results Distant peritoneal micrometastasis in rectovesical pouch or Douglas pouch were found in 10 cases out of the 40 cases , all of which were found to have full-thickness invasion or invasion out side gast ric serous tunic 〔27. 8 % (10/ 36) 〕. Their occurrence rates of peritoneal micrometastasis were significantly higher than those without full-thickness invasion〔0 (0/ 4) 〕, Plt;0. 05. The number of metastatic lymph nodes was more than six in 8 cases , was only one in 2 case , the occurrence rate of peritoneal micrometastasis of the number of metastatic lymph nodes was more than seven 〔44. 4 %(8/ 18) 〕which was significantly higher than that the number was less than seven〔16. 7 % (2/ 12) 〕, Plt;0. 05. In 10 cases , 8 cases were poorly differentiated adenocarcinoma , and the other two were moderately differentiated. Conclusion When gast ric carcinoma invaded serous tunic or outside , though peritoneal metastasis may not be found by preoperational inspection or intraoperative palpation , peritoneal biopsy in rectovesical pouch or Douglas pouch may be necessary to perform as a routine procedure to detect distant peritoneal micrometastasis. It may be useful for staging , adjuvant chemotherapy and prognosis forecast.
【Abstract】Objective To study the CT features of peritoneal Metastasis in postoperative patients of ovarian carcinomas. Methods CT appearance of peritoneal metastasis of ovarian carcinomas proved by surgery and pathology in 33 postoperative patients were reviewed. The CT features of the foci were recorded and analyzed, especially on the location, quantity, density and size.Results In the peritoneal cavity, 186 implant foci and 10 recurrent foci were found. metastasis often occurred in the right upper abdomen, especially the right subphrenic spaces. The most frequent locations were the right suprahepatic and subhepatic spaces, the small bowel mesentery, the gastrocolic ligament and the omentum. The density of the foci was most of solid. The size was ranged from 0.5~13 cm. Conclusion Peritoneal metastasis is the most frequent route of metastases for ovarian carcinomas. It is frequently found in upper abdomen, especially in the subphrenic spaces. Localized ascites in the peritoneal cavity is another important sign suggesting peritoneal implants. CT scan from the diaphragm to the pelvic floor will be helpful to diagnose peritoneal implants in cases of postoperative ovarian carcinomas.
Objective To evaluate the use value of the perineal H-shaped incision in laparoscopic peritoneum vaginoplasty. Methods Between January 2005 and July 2011, 29 cases of congenital absence of vagina underwent laparoscopic peritoneum vaginoplasty. There were 2 married cases and 27 unmarried cases, with a mean age of 22.7 years (range, 20-30 years). The patients had no vagina mouth or had a shallow depression. B ultrasound showed normal bilateral ovarian and no uterine or primordial uterus in the pelvic cavity. H-shaped incision was made between urethra and rectum for peritoneum vaginoplasty. Results All operations were successfully completed. The mean operation time was 130.5 minutes, and the mean blood loss was 73.5 mL. Primary healing of incision was achieved in all patients and no complication occurred. All the patients were followed up 1-2 years (mean, 1.5 years). At 3 months after operation, the mean length of neovagina was 9.5 cm (range, 8.2-10.5 cm); the neovagina could hold 2 fingers and the mucosa appeared so soft and smooth with normal lubrication; vaginal exfoliate cell maturation index was 50.5% ± 9.3% ; and the mean urethra-vaginal orifices spacing was 1.0 cm (range, 0.8-1.2 cm). At 1 year after operation, the mean length of the neovagina was 9.3 cm (range, 7.2-10.0 cm); the neovagina could hold 2 fingers; the vaginal exfoliate cell maturation index was 58.6% ± 8.1%; the mean urethra-vaginal orifices spacing was 1.1 cm (range, 0.9-1.3 cm). The rate of sexual satisfaction was 95%. Conclusion Use of the H-shaped incision in laparoscopic peritoneum vaginoplasty is a preferred way to reconstruct vagina for simple operation and good effectiveness.
In 14 dogs, the defect of the abdominal aorta was replaced by 5 to 8.5cm autogen ous peritoneal tube wich was composed of the peritoneum with the posterior sheath of the rectus muscle. The biophysical properties of the peritoneal tube was studied before and after replacement, and it showed that: (1) there was no significant difference between the arterial pressure of grafts and mormal abdominal aorts; (2) the tension strenghth was more than abdominal aorta; (3) the rate of patercy was 100 per cent. Therfore, the autogenous peritoneal tube might serve as a new type of material for replacement of vessels.