ObjectiveTo explore the risk factors and protective measures of the occupation exposure of probationer nurses in operation room. MethodsThe self-designed questionnaire was used to reveal the risk factors and protective measures of occupation exposure in probationer nurses worked at the operating room during January 2011 and December 2013. ResultsThe female probationer nurses in operating room accounted for 97.7%; the probationer nurses from the secondary specialized school accounted for 53.1%. All the nurses didn't received any occupational protection knowledge training in schools. The practical nurses who failed to follow the regulate operation accounted for 76.8%. The occupational exposure occurred in 457 nurses during the period, which accounted for 44.7% of all the probationer nurses surveyed; the biological occupational exposure accounted for 38.5%. ConclusionTo strengthen the occupation safety education of operation room nurses, strictly regulate the operation procedures, establish the consummation protection measures and regulations, and strengthen their self-protection awareness can effectively reduce the occupation exposure injury in the probationer nurses in operation room.