Objective To understand the adult male’s acceptance of circumcision and its influencing factors in Chongqing, so as to provide scientific evidences for developing male circumcision extension programs and health education materials. Methods The circumcision acceptance of adult males from 18 to 45 years old in Chongqing was investigated by using multistage stratified cluster sampling method. Results Among 1 502 subjects, 661 males persisted in their willingness to do circumcision, and the operation acceptance rate was 44.01%; the acceptance rate in the 18-30 age group was higher than that in the 31-45 age group; the higher the education level, the higher the acceptance of the circumcision; and workers and businessmen had greater willingness to accept than farmers. The multivariate logistic regression analysis showed that the positive factors affecting acceptance rate were “being aware that the circumcision is suitable for phimosis and redundant prepuce” (OR=21.01, 95%CI 14.21 to 31.06), “being told by friends who had circumcision before” (OR=1.65, 95%CI 1.25 to 2.19), and “being realized on the risk of phimosis and redundant prepuce” (OR=1.43, 95%CI 1.01 to 2.02); while the negative factors were “being worried about the surgical complications” (OR=0.69, 95%CI 0.50 to 0.95) and “being afraid of the ridicule from people around” (OR=0.63, 95%CI 0.40 to 0.99). Conclusion The willingness rate of adult males to accept circumcision was low in Chongqing; the publicity and education should be strengthened, and the good social atmosphere should be built for increasing the willingness rate of accepting circumcision.