In order to study the biological properties of fibroblasts isolated from different tissues. The fibroblasts from normal skin, hypertrophic scar and keloid were cultured, respectively, in vitro, and their morphologies and growth kinetics were compared. The results revealed that although fibroblasts in keloid were irregularly arranged, crisscross and overlapping with loss of polarization, there was no significant difference in the 3 groups so far the cellular morphology of fibroblast itself, cellular growth curve, cellular mitotic index, cloning efficiency and DNA content provided those cultures were in the same cellular density and culture conditions. It was concluded that fibroblasts isolated from culture of normal skin, hypertrophic scar and keloid in vitro showed no significant difference in morphology and growth kinetics, on the contrary, their biological behaviors were quite similar.
In experimental study on pigs was designed to evaluate the changes of the vessel density of super thin skin flaps.By means of stratified sampling, isotropic uniform random section, the vessel density of the supor thin skin flap was obeerved under the MIAS-200 image analyzer. The results showed that the remarkable changes of vascular density were found to begin on the 5th day after operation and was first appeared in the distal port of the survived flap,then,it proceeded gradually from the distal proximally.